Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
KLH-conjugated, full-length ubiquitin isolated from bovine erythrocytes.
Specificity
Recognizes ubiquitin, Mr ~8kD, and ubiquitinylated proteins. Species cross-reactivity: Bovine and human. Broad species cross-reactivity is expected based on sequence homology.
Applications
- Western blot (0.5 - 2 µg/ml)
Usage
Suitable for use in Western Blot. Western Blot Analysis: 0.5-2.0 ug/ml of this lot detected full-length ubiquitin from bovine erythrocytes, and ubiquitinated proteins in acid extracts from HeLa cells subjected to high temperature antigen retrieval.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee