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Antibodies > UBE3B > LS-C801857
 
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Catalog Number Size Price
LS-C801857-100 100 µl (1 mg/ml) $379 
LS-C801857-200 200 µl (1 mg/ml) $421 
UBE3B Antibody - 1:100 staining human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
UBE3B Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
UBE3B Antibody - Western blot analysis UBE3B using Jurkat whole cells lysates
UBE3B Antibody - 1:100 staining human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
UBE3B Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
UBE3B Antibody - Western blot analysis UBE3B using Jurkat whole cells lysates
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Polyclonal Rabbit anti‑Human UBE3B Antibody (IHC, IF, WB) LS‑C801857

Polyclonal Rabbit anti‑Human UBE3B Antibody (IHC, IF, WB) LS‑C801857

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Antibody:
UBE3B Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801857-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
UBE3B Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
UBE3B antibody LS-C801857 is an unconjugated rabbit polyclonal antibody to human UBE3B. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human UBE3B
Synonyms
UBE3B | Ubiquitin-protein ligase E3B | Ubiquitin protein ligase E3B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
Synthesized peptide derived from human UBE3B
Specificity
UBE3B Antibody detects endogenous levels of total UBE3B.
Applications
  • IHC
  • Immunofluorescence (1:100 - 1:500)
  • Western blot
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About UBE3B
Q7Z3V4 Q7Z3V4

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Species: Human
Applications: IHC, Western blot, ELISA
UBE3B Antibody - Immunohistochemical analysis of UBE3B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
UBE3B Antibody (Internal) LS‑C368937
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
UBE3B Antibody - Western blot analysis of lysates from Jurkat cells, using UBE3B Antibody. The lane on the right is blocked with the synthesized peptide.
UBE3B Antibody (aa581‑630) LS‑C120456
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
UBE3B Antibody - UBE3B antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
UBE3B Antibody (aa720‑769) LS‑C102027
Species: Human
Applications: Western blot
UBE3B Antibody - UBE3B antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
UBE3B Antibody (aa720‑769, Biotin) LS‑C445077
Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken
Applications: Western blot
UBE3B Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Human UBE3B Protein (Recombinant GST, N-terminus) (aa118-225) - LS-G63456
Source: Wheat
Tag: GST, N-terminus
See all UBE3B Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/2/2024
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