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Catalog Number Size Price
LS-C817546-100 100 µl $412 
UBE2M / UBC12 Antibody - Anti-UBE2M rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: HEK-293 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: U251MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa.
UBE2M / UBC12 Antibody - UBE2M was immunoprecipitated using: Lane A: 0.5 mg HEK-293 Whole Cell Lysate. 4 uL anti-UBE2M rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UBE2M rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa.
UBE2M / UBC12 Antibody - Anti-UBE2M rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: HEK-293 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: U251MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa.
UBE2M / UBC12 Antibody - UBE2M was immunoprecipitated using: Lane A: 0.5 mg HEK-293 Whole Cell Lysate. 4 uL anti-UBE2M rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UBE2M rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa.
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Polyclonal Rabbit anti‑Human UBE2M / UBC12 Antibody (WB) LS‑C817546

Polyclonal Rabbit anti‑Human UBE2M / UBC12 Antibody (WB) LS‑C817546

Antibody:
UBE2M / UBC12 Rabbit anti-Human Polyclonal Antibody
Application:
WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$412
LS-C817546-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
UBE2M / UBC12 Rabbit anti-Human Polyclonal Antibody
Application:
WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
UBC12 antibody LS-C817546 is an unconjugated rabbit polyclonal antibody to human UBC12 (UBE2M). Validated for IP and WB.
Target
Human UBE2M / UBC12
Synonyms
UBE2M | NEDD8 protein ligase | NEDD8 carrier protein | NEDD8-conjugating enzyme Ubc12 | HUbc12 | UBC-RS2 | UBC12 | Ubiquitin-protein ligase M | Yeast UBC12 homolog | Ubiquitin carrier protein M
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
E. coli-derived Human UBE2M fragment
Specificity
Human UBE2M
Applications
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.0, 0.03% Proclin 300
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About UBE2M / UBC12
P61081 NM_003969 NP_003960.1

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Requested From: United States
Date Requested: 4/28/2024