Target
Human UBC6 / UBE2J2
Synonyms
UBE2J2 | NCUBE2 | Ubc6p | Ubiquitin conjugating enzyme 6 | NCUBE-2 | Yeast UBC6 homolog | PRO2121 | UBC6
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Predicted
Human, Mouse, Rat, Bovine, Dog, Horse, Opossum, Chicken, Platypus, Zebra finch, Xenopus, Salmon, Macaque (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the human Ube2j2 protein.
Specificity
A BLAST analysis was used to suggest cross-reactivity with Ube2j2 protein from human, horse, opossum, cattle, dog, mouse, rat, macaque, salmon, chicken, zebra finch, Xenopus and platypus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
- Western blot (1:500 - 1:3000)
- Immunoprecipitation
- ELISA (1:5000 - 1:25000)
Usage
This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29 kD in size corresponding to Ube2j2 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee