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Catalog Number Size Price
LS-C39952-100 100 µg $806 
Antibody

Polyclonal Sheep anti‑Human Trypsin 1,2,3 (PRSS1,2,3) Antibody (aa16‑247, WB) LS‑C39952

Polyclonal Sheep anti‑Human Trypsin 1,2,3 (PRSS1,2,3) Antibody (aa16‑247, WB) LS‑C39952

Antibody:
Trypsin 1,2,3 (PRSS1,2,3) Sheep anti-Human Polyclonal (aa16-247) Antibody
Application:
WB, IP, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$806
LS-C39952-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Trypsin 1,2,3 (PRSS1,2,3) Sheep anti-Human Polyclonal (aa16-247) Antibody
Application:
WB, IP, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Trypsin 1,2,3 (PRSS1,2,3) antibody LS-C39952 is an unconjugated sheep polyclonal antibody to human Trypsin 1,2,3 (PRSS1,2,3) (aa16-247). Validated for ELISA, IP, Neut and WB.
Target
Human Trypsin 1,2,3 (PRSS1,2,3)
Host
Sheep
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
NS0-derived rhTrypsin 2 (aa16-247).
Epitope
aa16-247
Specificity
Selected for its ability to recognize human Trypsin 1, human Trypsin 2, and human Trypsin 3 in direct ELISAs and Western Blots.
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • Immunoprecipitation
  • ELISA (0.5 - 1 µg/ml)
  • Neutralization
Usage
Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human Trypsin 1/2/3. The detection limit for rhTrypsin 1/2/3 is approximately 1 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: Used to immunoprecipitate rhTrypsin 1/2/3 from conditioned media of transfected NS0 cells. Neutralization: Used to inhibit/neutralize the enzymatic activity of rhTrypsin 2. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 0.3 ug/ml (IC50) under conditions in which the enzyme was present at 0.02 ug/ml and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26 kD) and the antibody (150 kD), IC50 was achieved at approximately 3:1 molar ratio of the antibody to the enzyme. The antibody did not inhibit the activity of rhTrypsin 1 and rhTrypsin 3 significantly. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Trypsin 1/2/3. The detection limit for rhTrypsin 1/2/3 is approximately 0.8 ng/well.
Presentation
Lyophilized
Reconstitution
Reconstitute in deionized water
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/17/2021