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Catalog Number Size Price
LS-C39952-100 100 µg $785 
Antibody

Trypsin 1,2,3 (PRSS1,2,3) Antibody (aa16‑247) LS‑C39952

Trypsin 1,2,3 (PRSS1,2,3) Antibody (aa16‑247) LS‑C39952

Antibody:
Sheep Polyclonal to Human Trypsin 1,2,3 (PRSS1,2,3)
Application:
WB, IP, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$785 
LS-C39952-100
100 µg
Toll Free North America
866-819-4732

Overview

Antibody:
Sheep Polyclonal to Human Trypsin 1,2,3 (PRSS1,2,3)
Application:
WB, IP, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Trypsin 1,2,3 (PRSS1,2,3) antibody LS-C39952 is an unconjugated sheep polyclonal antibody to human Trypsin 1,2,3 (PRSS1,2,3). Validated for ELISA, IP, Neut and WB.
Target
Human Trypsin 1,2,3 (PRSS1,2,3)
Host
Sheep
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • Immunoprecipitation
  • ELISA (0.5 - 1 µg/ml)
  • Neutralization
Immunogen
NS0-derived rhTrypsin 2 (aa16-247).
Epitope
aa16-247
Specificity
Selected for its ability to recognize human Trypsin 1, human Trypsin 2, and human Trypsin 3 in direct ELISAs and Western Blots.
Usage
Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human Trypsin 1/2/3. The detection limit for rhTrypsin 1/2/3 is approximately 1 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: Used to immunoprecipitate rhTrypsin 1/2/3 from conditioned media of transfected NS0 cells. Neutralization: Used to inhibit/neutralize the enzymatic activity of rhTrypsin 2. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 0.3 ug/ml (IC50) under conditions in which the enzyme was present at 0.02 ug/ml and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26 kD) and the antibody (150 kD), IC50 was achieved at approximately 3:1 molar ratio of the antibody to the enzyme. The antibody did not inhibit the activity of rhTrypsin 1 and rhTrypsin 3 significantly. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Trypsin 1/2/3. The detection limit for rhTrypsin 1/2/3 is approximately 0.8 ng/well.
Presentation
Lyophilized
Reconstitution
Reconstitute in deionized water
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/14/2019