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Catalog Number Size Price
LS-C59554-100 100 µg (2.4 mg/ml) $498 
TP53BP2 / ASPP2 Antibody - Anti-ASPP2 Antibody - Western Blot. Western blot of Protein A purified anti-ASPP2 to detect over-expressed ASPP2 in MCF-7 cells (Lane 2, arrowhead). Lane 1 is a nontransfected control. Lane 3 is MCF-7 cells over-expressing ASPP1. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1000 dilution. The identity of the lower MW band at approximately 40kD is unknown. Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.

Polyclonal Rabbit anti‑Human TP53BP2 / ASPP2 Antibody (IHC, WB) LS‑C59554

Polyclonal Rabbit anti‑Human TP53BP2 / ASPP2 Antibody (IHC, WB) LS‑C59554

Antibody:
TP53BP2 / ASPP2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C59554-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TP53BP2 / ASPP2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ASPP2 antibody LS-C59554 is an unconjugated rabbit polyclonal antibody to human ASPP2 (TP53BP2). Validated for ELISA, IHC and WB.
Target
Human TP53BP2 / ASPP2
Synonyms
TP53BP2 | 53BP2 | p53-binding protein 53bp2 | p53BP2 | PPP1R13A | ASPP2 | BBP | BCL2-binding protein | p53-binding protein 2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Dog, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP2.
Specificity
This Protein A purified antibody is directed against human ASPP2. Minimal reactivity occurs against ASPP1. A BLAST analysis was used to suggest cross-reactivity with ASPP2 from macaque and dog sources based on a 100% homology with the immunizing sequence.  Expect partial reactivity with ASPP2 from mouse and rat sources based on an 80% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Applications
  • IHC
  • Western blot (1:500 - 1:3000)
  • ELISA (1:2500 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kD in size corresponding to ASPP2 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53BP2 / ASPP2
Q13625 NM_005426 NP_005417.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/10/2022