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Catalog Number Size Price
LS-C761936-100 100 µg (1 mg/ml) $574 
TOP2A / Topoisomerase II Alpha Antibody - IHC testing of FFPE human bladder carcinoma with Topoisomerase II alpha antibody (clone TOP2A/1361). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
TOP2A / Topoisomerase II Alpha Antibody - IHC testing of FFPE human tonsil with Topoisomerase II alpha antibody (clone TOP2A/1361). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
TOP2A / Topoisomerase II Alpha Antibody - Western blot testing of 1) human HepG2, 2) HeLa and 3) mouse NIH3T3 cell lysate with Topoisomerase II alpha antibody (clone TOP2A/1361). Expected molecular weight ~174 kDa.
TOP2A / Topoisomerase II Alpha Antibody - IHC testing of FFPE human bladder carcinoma with Topoisomerase II alpha antibody (clone TOP2A/1361). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
TOP2A / Topoisomerase II Alpha Antibody - IHC testing of FFPE human tonsil with Topoisomerase II alpha antibody (clone TOP2A/1361). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
TOP2A / Topoisomerase II Alpha Antibody - Western blot testing of 1) human HepG2, 2) HeLa and 3) mouse NIH3T3 cell lysate with Topoisomerase II alpha antibody (clone TOP2A/1361). Expected molecular weight ~174 kDa.
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Monoclonal Mouse anti‑Human TOP2A / Topoisomerase II Alpha Antibody (clone TOP2A/1361, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C761936

Monoclonal Mouse anti‑Human TOP2A / Topoisomerase II Alpha Antibody (clone TOP2A/1361, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C761936

Antibody:
TOP2A / Topoisomerase II Alpha Mouse anti-Human Monoclonal (Azide-free, BSA-free) (TOP2A/1361) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Price
Catalog Number
$574
LS-C761936-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TOP2A / Topoisomerase II Alpha Mouse anti-Human Monoclonal (Azide-free, BSA-free) (TOP2A/1361) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:

Specifications

Description
Topoisomerase II Alpha antibody LS-C761936 is an unconjugated mouse monoclonal antibody to human Topoisomerase II Alpha (TOP2A). Validated for Flow, IF, IHC and WB.
Target
Human TOP2A / Topoisomerase II Alpha
Synonyms
TOP2A | DNA topoisomerase II, 170 kD | TOP2 | DNA gyrase | DNA topoisomerase 2-alpha | TP2A | Topoisomerase IIa
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
TOP2A/1361
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Azide-free, BSA-free. Also available Unmodified.
Immunogen
Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.
Presentation
1X PBS
Storage
Store at 2-8°C (with azide) or aliquot and store at -20°C (without azide).
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TOP2A / Topoisomerase II Alpha
P11388 NM_001067 NP_001058.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024