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Catalog Number Size Price
LS-C662574-10 10 µg $318 
LS-C662574-100 100 µg $470 
TNFRSF13B / TACI Antibody - Western blot analysis of TACI using anti-TACI antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TACI protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACI antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TACI at approximately 40KD. The expected band size for TACI is at 32KD.
TNFRSF13B / TACI Antibody - Western blot - Anti-TACI antibody
TNFRSF13B / TACI Antibody - Western blot analysis of TACI using anti-TACI antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TACI protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACI antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TACI at approximately 40KD. The expected band size for TACI is at 32KD.
TNFRSF13B / TACI Antibody - Western blot - Anti-TACI antibody
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Polyclonal Rabbit anti‑Human TNFRSF13B / TACI Antibody (WB) LS‑C662574

Polyclonal Rabbit anti‑Human TNFRSF13B / TACI Antibody (WB) LS‑C662574

Antibody:
TNFRSF13B / TACI Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662574-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TNFRSF13B / TACI Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TACI antibody LS-C662574 is an unconjugated rabbit polyclonal antibody to human TACI (TNFRSF13B). Validated for ELISA and WB.
Target
Human TNFRSF13B / TACI
Synonyms
TNFRSF13B | CD267 antigen | CD267 | CVID2 | TNFRSF14B | CVID | TACI
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human TACI recombinant protein (Position: M1-S165).
Specificity
Highly expressed in spleen, thymus, small intestine and peripheral blood leukocytes. Expressed in resting B- cells and activated T-cells, but not in resting T-cells.
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNFRSF13B / TACI
O14836 NM_012452 NP_036584.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024