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Catalog Number Size Price
LS-C745272-25 25 µl (1.15 mg/ml) $304 
TNF Alpha Antibody - Western blot using the protein-A purified anti-swine TNFa antibody shows detection of recombinant Swine TNFa at about 16.9 kDa raised in yeast. The identity of the faint band at 135 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with for 45 min at room temperature.

Polyclonal Rabbit anti‑Pig TNF Alpha Antibody (Liquid, IHC, WB) LS‑C745272

Polyclonal Rabbit anti‑Pig TNF Alpha Antibody (Liquid, IHC, WB) LS‑C745272

Antibody:
TNF Alpha Rabbit anti-Pig Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Pig
Format:
Unconjugated, Liquid
Price
Catalog Number
$304
LS-C745272-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TNF Alpha Rabbit anti-Pig Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Pig
Format:
Unconjugated, Liquid

Specifications

Description
TNF Alpha antibody LS-C745272 is an unconjugated rabbit polyclonal antibody to pig TNF Alpha. Validated for ELISA, IHC and WB.
Target
Pig TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Host
Rabbit
Reactivity
Pig (tested or 100% immunogen sequence identity)
Predicted
Baboon (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Liquid
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNFa.
Specificity
A BLAST analysis was used to suggest cross-reactivity with TNFa from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against TNFa from baboon based on 90% homology. Cross-reactivity with TNFa from sources other than swine has not been determined.
Applications
  • IHC
  • Western blot (1:1000)
  • ELISA (1:2000 - 1:2500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNF Alpha
P01375 NM_000594 NP_000585.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/7/2024