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Catalog Number Size Price
LS-C104683-500 500 µg $326 
TNF Alpha Antibody - Anti-Human TNF-a Western Blot Unreduced
TNF Alpha Antibody - Anti-Human TNF-a Sandwich ELISA
TNF Alpha Antibody - Anti-Human TNF-a Western Blot Unreduced
TNF Alpha Antibody - Anti-Human TNF-a Sandwich ELISA
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Monoclonal Mouse anti‑Human TNF Alpha Antibody (WB) LS‑C104683

Monoclonal Mouse anti‑Human TNF Alpha Antibody (WB) LS‑C104683

Note: This antibody replaces LS-C54331
Antibody:
TNF Alpha Mouse anti-Human Monoclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$326
LS-C104683-500
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TNF Alpha Mouse anti-Human Monoclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TNF Alpha antibody LS-C104683 is an unconjugated mouse monoclonal antibody to human TNF Alpha. Validated for ELISA and WB.
Target
Human TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human TNF-alpha.
Specificity
Human TNF-alpha
Applications
  • Western blot (0.5 - 1 µg/ml)
  • ELISA (2 - 4 µg/ml)
Usage
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 15-20 ug/ml overnight at 4°C. An HRP-labeled polymer detection system was used with DAB Chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. *Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-alpha (0.5 ng/ml), a concentration of < 0.1 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human TNF-alpha when used with biotinylated antigen affinity purified anti-Human TNF-alpha (LS-C104719) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect Human TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.5-1 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-alpha is 0.5-1 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute in sterile water to 1 mg/ml.
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNF Alpha
P01375 NM_000594 NP_000585.2

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Request SDS/MSDS

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Requested From: United States
Date Requested: 6/14/2024