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Catalog Number	Size	Price
LS-C355488-1	1 mg	$503

TNF Alpha Antibody - Direct ELISA analysis of TNF alpha was performed by coating wells of a 96-well plate with 100ul per well of human recombinant protein TNF alpha diluted in carbonate/bicarbonate buffer at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with starting blocking buffer, and incubated with 100ul per well of a rabbit anti-TNF alpha polyclonal antibody at a dilution of 1:25, 1:50, 1:100, 1:200, 1:400, 1:800, 1:1600 and 1:3200 for 90 minutes at 37C. The plate was washed, then incubated with 100ul per well of a HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:2000 for 90 minutes at 37C. Detection was performed using 1-Step Ultra TMB substrate for 5-10 minutes at room temperature in the dark. The reaction was stopped with Stop solution, and absorbances were read on a spectrophotometer at 450-550nm.

Polyclonal Rabbit anti‑Human TNF Alpha Antibody (IHC, WB) LS‑C355488

Name: Anti-TNF Alpha Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C355488-1
Price: 503.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

TNF Alpha Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB, ELISA, Neut

Reactivity:

Human

Format:

Unconjugated, Unmodified

$503

LS-C355488-1

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

TNF Alpha Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB, ELISA, Neut

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

TNF Alpha antibody LS-C355488 is an unconjugated rabbit polyclonal antibody to human TNF Alpha. Validated for ELISA, IHC, Neut and WB.

Target

Human TNF Alpha

Synonyms

See All TNF Alpha Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Protein G purified

Modifications

Unmodified

Immunogen

Recombinant human TNF alpha

Specificity

The antibody targets TNF Alpha in ELISA, IHC(P), Neu, and WB applications and shows reactivity with Human samples. It detects TNF Alpha which has a predicted molecular weight of approximately 26 kD.

Applications

IHC
IHC - Paraffin
Western blot
ELISA
Neutralization

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, no preservative.

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About TNF Alpha

Uniprot: P01375 NCBI: NM_000594 NP_000585.2