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Catalog Number Size Price
LS-C59380-1 1 ml (75 mg/ml) $964 
TNF Alpha Antibody - Anti-Human TNFa Antibody - Immunohistochemistry. Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4°C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.
TNF Alpha Antibody - Anti-Human TNFa Antibody - Immunohistochemistry. Fluorescent immunohistochemistry showing staining of human colon by anti-TNF alpha (formalin/PFA-fixed paraffin-embedded sections). Samples were formaldehyde-fixed, then blocked in 10% serum for 2 hours at 20°C. The primary antibody was diluted 1:100 and incubated with the sample for 2 hours at 20°C. Alexa Fluor 680 goat polyclonal secondary antibody was used diluted 1:5000.
TNF Alpha Antibody - Anti-Human TNF-a Antibody - Western Blot. Western blot of Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4C, DyLight 649 Gt-a-Rb added at 1:20000 in MB-070 30 min RT.
TNF Alpha Antibody - Anti-Human TNFa Antibody - Immunohistochemistry. Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4°C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.
TNF Alpha Antibody - Anti-Human TNFa Antibody - Immunohistochemistry. Fluorescent immunohistochemistry showing staining of human colon by anti-TNF alpha (formalin/PFA-fixed paraffin-embedded sections). Samples were formaldehyde-fixed, then blocked in 10% serum for 2 hours at 20°C. The primary antibody was diluted 1:100 and incubated with the sample for 2 hours at 20°C. Alexa Fluor 680 goat polyclonal secondary antibody was used diluted 1:5000.
TNF Alpha Antibody - Anti-Human TNF-a Antibody - Western Blot. Western blot of Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4C, DyLight 649 Gt-a-Rb added at 1:20000 in MB-070 30 min RT.
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Polyclonal Rabbit anti‑Human TNF Alpha Antibody (IHC, IF, WB) LS‑C59380

Polyclonal Rabbit anti‑Human TNF Alpha Antibody (IHC, IF, WB) LS‑C59380

Antibody:
TNF Alpha Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Primate
Format:
Unconjugated, Unmodified
Price
Catalog Number
$964
LS-C59380-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TNF Alpha Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Primate
Format:
Unconjugated, Unmodified

Specifications

Description
TNF Alpha antibody LS-C59380 is an unconjugated rabbit polyclonal antibody to TNF Alpha from human. It is reactive with human and primate. Validated for ELISA, IF, IHC and WB.
Target
Human TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Host
Rabbit
Reactivity
Human, Primate (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
The whole rabbit serum was prepared by repeated immunizations with recombinant human TNFa produced in E.coli.
Specificity
The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFb (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Applications
  • IHC (1:100 - 1:500)
  • Immunofluorescence (1:100)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:1000 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This antiserum against Human TNFa has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. It recognizes the 17000 MW TNFa. Reactivity in other immunoassays is unknown.
Presentation
Antiserum
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNF Alpha
P01375 NM_000594 NP_000585.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/8/2022