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Catalog Number Size Price
LS-C104810-25 25 µg $356 
LS-C104810-50 50 µg $396 
TNF Alpha Antibody - Sandwich ELISA of TNF Alpha / TNF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Goat anti‑Mouse TNF Alpha Antibody (Biotin, WB) LS‑C104810

Polyclonal Goat anti‑Mouse TNF Alpha Antibody (Biotin, WB) LS‑C104810

Note: This antibody replaces LS-C54305, LS-C39991
Antibody:
TNF Alpha Goat anti-Mouse Polyclonal (Biotin) Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Biotin, Unmodified
Other formats:
Price
Catalog Number
$356
LS-C104810-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TNF Alpha Goat anti-Mouse Polyclonal (Biotin) Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Biotin, Unmodified
Other formats:

Specifications

Description
TNF Alpha antibody LS-C104810 is a biotin-conjugated goat polyclonal antibody to mouse TNF Alpha. Validated for ELISA and WB.
Target
Mouse TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Murine TNF-alpha.
Specificity
Mouse TNF-alpha
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.25 - 1 µg/ml)
  • Applications tested for the base form of this product only
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Murine TNF-alpha by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine TNF-alpha. To detect Murine TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine TNF-alpha (LS-C104784) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine TNF-alpha. Western Blot: To detect Murine TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine TNF-alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile PBS containing 0.1% BSA
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNF Alpha
P01375 NM_000594 NP_000585.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/15/2024