Human TNC / Tenascin C

Rabbit

Human (tested or 100% immunogen sequence identity)

Rat

IgG Recombinant Monoclonal

TNC/2981R

Unconjugated

Protein A/G purified

Azide-free, Carrier-free. Also available Unmodified.

Recombinant full-length human TNC protein. Antigen Molecular Weight: 210 kDa and 300 kDa

In Western blotting, it reacts with two bands of ~MW of 210 kDa and 300 kDa , identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

• IHC - Paraffin (2 - 4 µg/ml)

Immunohistology (Formalin-fixed - 2-4 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

1 mM PBS

Store at -20°C to -80°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P24821 NCBI: NM_002160 NP_002151.2