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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TMF1 / TMF‑1 Antibody LS‑C681577
TMF-1 antibody LS-C681577 is an unconjugated rabbit polyclonal antibody to human TMF-1 (TMF1). Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C681577-50
50 µg
$285
LS-C681577-100
100 µg
$385

Popular TMF1 / TMF-1 Products

TMF1 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine, Dog, Guinea pig, Horse
Applications: Western blot
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

TMF-1 antibody LS-C681577 is an unconjugated rabbit polyclonal antibody to human TMF-1 (TMF1). Validated for ELISA, IHC and WB.

Specifications

Target
Human TMF1 / TMF-1
Synonyms
TMF1, TMF, ARA160, TATA element modulatory factor
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TMF1 / TMF-1 antibody was raised against recombinant Human TATA element modulatory factor protein (878-986AA)
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only.
About TMF1 / TMF-1
Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). P82094 NM_007114 NP_009045.2


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Images

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Western blot

Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All Lanes: TMF1 antibody at 3.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 123, 124 KDa Observed band size: 123, 124 KDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All Lanes: TMF1 antibody at 3.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 123, 124 KDa Observed band size: 123, 124 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Western blot

Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All Lanes: TMF1 antibody at 3.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 123, 124 KDa Observed band size: 123, 124 KDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All Lanes: TMF1 antibody at 3.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 123, 124 KDa Observed band size: 123, 124 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Western blot

Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All Lanes: TMF1 antibody at 3.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 123, 124 KDa Observed band size: 123, 124 KDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All Lanes: TMF1 antibody at 3.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 123, 124 KDa Observed band size: 123, 124 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Western blot

Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All Lanes: TMF1 antibody at 3.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 123, 124 KDa Observed band size: 123, 124 KDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All Lanes: TMF1 antibody at 3.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 123, 124 KDa Observed band size: 123, 124 KDa

Requested From: United States
Date Requested: 11/16/2018

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