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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TMF1 / TMF‑1 Antibody (aa1008‑1057) LS‑C112343
TMF-1 antibody LS-C112343 is an unconjugated rabbit polyclonal antibody to TMF-1 (TMF1) from human, bat, horse and other species. Validated for WB.
Catalog
Size
Price
LS-C112343-50
50 µg
$365

Popular TMF1 / TMF-1 Products

TMF1 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine, Dog, Guinea pig, Horse
Applications: Western blot
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA
Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

TMF-1 antibody LS-C112343 is an unconjugated rabbit polyclonal antibody to TMF-1 (TMF1) from human, bat, horse and other species. Validated for WB.

Specifications

Target
Human TMF1 / TMF-1
Synonyms
TMF1, TMF, ARA160, TATA element modulatory factor
Host
Rabbit
Reactivity
Human, Monkey, Bat, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
TMF1 / TMF-1 antibody was raised against synthetic peptide located between aa1008-1057 of human TMF1 (P82094, NP_009045). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Panda, Bat, Rabbit, Horse, Pig (100%); Mouse, Rat, Hamster, Bovine, Opossum, Guinea pig, Zebra finch, Lizard, Xenopus (92%); Turkey, Chicken (85%).
Blocking Peptide
LS-E10808 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C112343. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1008-1057
Specificity
Human TMF1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About TMF1 / TMF-1
Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). P82094 NM_007114 NP_009045.2


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Images

Western blot

TMF1 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TMF1 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TMF1 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TMF1 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TMF1 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TMF1 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TMF1 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TMF1 antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/15/2018

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