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Catalog Number Size Price
LS-C773825-100 100 µg (1 mg/ml) $474 
TMEM49 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-VMP1 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane, Vacuole Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VMP1 Antibody. (D) Composite.
TMEM49 Antibody - Western blot analysis of Human A549 showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: A549 cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa. Other Band(s): Multiple bands for A549; Rat Brain shows a band at 60kDa, 75kDa, and 150kDa.
TMEM49 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-VMP1 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane, Vacuole Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VMP1 Antibody. (D) Composite.
TMEM49 Antibody - Western blot analysis of Human A549 showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: A549 cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa. Other Band(s): Multiple bands for A549; Rat Brain shows a band at 60kDa, 75kDa, and 150kDa.
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Polyclonal Rabbit anti‑Human TMEM49 Antibody (AP, IF, WB) LS‑C773825

Polyclonal Rabbit anti‑Human TMEM49 Antibody (AP, IF, WB) LS‑C773825

Antibody:
TMEM49 Rabbit anti-Human Polyclonal (AP) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Alkaline Phosphatase, Unmodified
Other formats:
Price
Catalog Number
$474
LS-C773825-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TMEM49 Rabbit anti-Human Polyclonal (AP) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Alkaline Phosphatase, Unmodified
Other formats:

Specifications

Description
TMEM49 antibody LS-C773825 is an AP-conjugated rabbit polyclonal antibody to human TMEM49. Validated for IF and WB.
Target
Human TMEM49
Synonyms
VMP1 | TMEM49 | TANGO5 | Transmembrane protein 49 | EPG3 | TDC1 | Vacuole membrane protein 1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Alkaline Phosphatase. Also available conjugated with APC, Atto 390, Atto 488, Atto 594, Biotin, FITC, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the C-Terminus of Human VMP1 (aa. 391-402)
Specificity
Detects ~46 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TMEM49
Q96GC9 NM_030938 NP_112200.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/28/2024