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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TLR4 Antibody (aa100‑150) LS‑C845
TLR4 antibody LS-C845 is an unconjugated rabbit polyclonal antibody to TLR4 from human and mouse. Validated for IP and WB.
Catalog
Size
Price
LS-C845-100
100 µg (0.5 mg/ml)
$335

Popular TLR4 Products

Anti-TLR4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2070 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Anti-TLR4 antibody LS-A9704 IHC of human placenta, decidua. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin
Anti-TLR4 antibody IHC of human neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4696 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin
Anti-TLR4 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2254 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry, ELISA
Human peripheral blood monocytes stained with HTA125 PE
Species: Human, Guinea pig, Pig
Applications: IHC, IHC - Frozen, Immunofluorescence, Flow Cytometry

Product Description

TLR4 antibody LS-C845 is an unconjugated rabbit polyclonal antibody to TLR4 from human and mouse. Validated for IP and WB.

Specifications

Target
Human TLR4
Synonyms
TLR4, CD284, CD284 antigen, Homolog of Drosophila toll, TLR-4, TOLL, ARMD10, Toll-like receptor 4, HToll
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation (1:10 - 1:500)
Immunogen
TLR4 antibody was raised against a portion of amino acids 100-150 of human TLR4. It will cross-react with mouse TLR4.
Epitope
aa100-150
Specificity
Observed size: approx. 90 kD (native) or 80 kD (partial recombinant mouse TLR4--extracellular portion plus His-tag).
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About TLR4
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+. Responses triggered by Ni2+ require non-conserved histidines and are, therefore, species-specific. O00206 NM_138554 O00206


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Images

Western blot

Western blot of TLR4 using antibody on (A) 0.1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/ml), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/ml on intestine lysates).
Western blot of TLR4 using antibody on (A) 0.1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/ml), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/ml on intestine lysates).

Western blot

Western blot of TLR4 using antibody on (A) 0.1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/ml), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/ml on intestine lysates).
Western blot of TLR4 using antibody on (A) 0.1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/ml), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/ml on intestine lysates).

Western blot

Western blot of TLR4 using antibody on (A) 0.1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/ml), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/ml on intestine lysates).
Western blot of TLR4 using antibody on (A) 0.1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/ml), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/ml on intestine lysates).

Western blot

Western blot of TLR4 using antibody on (A) 0.1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/ml), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/ml on intestine lysates).
Western blot of TLR4 using antibody on (A) 0.1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/ml), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/ml on intestine lysates).

Requested From: United States
Date Requested: 10/16/2018

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