Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > TK1 / TK / Thymidine Kinase > LS-C757648
 
Print Page  
Catalog Number Size Price
LS-C757648-200 200 µl $393 
TK1 / TK / Thymidine Kinase Antibody - TK1 Antibody (Center) staining TK1 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
TK1 / TK / Thymidine Kinase Antibody - All lanes: Anti-TK1 Antibody (Center) at 1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
TK1 / TK / Thymidine Kinase Antibody - Overlay histogram showing U-2 OS cells stained with TK1 Antibody (Center) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (TK1 Antibody (Center), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
TK1 / TK / Thymidine Kinase Antibody - TK1 Antibody (Center) staining TK1 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
TK1 / TK / Thymidine Kinase Antibody - All lanes: Anti-TK1 Antibody (Center) at 1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
TK1 / TK / Thymidine Kinase Antibody - Overlay histogram showing U-2 OS cells stained with TK1 Antibody (Center) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (TK1 Antibody (Center), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human TK1 / TK / Thymidine Kinase Antibody (Internal, IHC, WB) LS‑C757648

Polyclonal Rabbit anti‑Human TK1 / TK / Thymidine Kinase Antibody (Internal, IHC, WB) LS‑C757648

Submit Review LSBio Guarantee
Antibody:
TK1 / TK / Thymidine Kinase Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC-P, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757648-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TK1 / TK / Thymidine Kinase Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC-P, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Thymidine Kinase antibody LS-C757648 is an unconjugated rabbit polyclonal antibody to human Thymidine Kinase (TK1 / TK) (Internal). Validated for ELISA, Flow, IHC and WB.
Target
Human TK1 / TK / Thymidine Kinase
Synonyms
TK1 | Spleen tyrosine kinase | Thymidine kinase 1, soluble | Thymidine Kinase 1 | Thymidine kinase, cytosolic | Thymidine kinase-1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Immunogen
This TK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 139-173 amino acids from the Central region of human TK1.
Epitope
Internal
Applications
  • IHC - Paraffin (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot Predicted Molecular Weight: 25.5 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TK1 / TK / Thymidine Kinase
P04183 NM_003258 NP_003249.3

Publications (0)

Submit a Publication for LS-C757648

Customer Reviews (0)

Submit a review for LS-C757648

Featured Products

Product
TK1 / TK / Thymidine Kinase Antibody (clone 3B3.E11) LS‑C83445
Species: Human
Applications: Western blot, ELISA
Product
TK1 / TK / Thymidine Kinase Antibody (130‑210 aa, C‑terminal) LS‑C386411
Species: Human, Monkey
Applications: Western blot, ELISA
TK1 / TK / Thymidine Kinase Antibody - Knockout Varification: Lane 1: Wild-type MCF7 cell lysate; Lane 2: TK1 knockout MCF7 cell lysate; Predicted MW: 25kDa Observed MW: 25kDa Primary Ab: 6µg/ml Mouse Anti-Human TK1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
TK1 / TK / Thymidine Kinase Antibody LS‑C663731
Species: Human, Mouse, Rat, Pig
Applications: ICC, Western blot, Immunoprecipitation
TK1 / TK / Thymidine Kinase Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TK Antibody. The picture on the right is blocked with the synthesized peptide.
TK1 / TK / Thymidine Kinase Antibody (aa1‑50) LS‑C117719
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Product
TK1 / TK / Thymidine Kinase Antibody (1‑80 aa) LS‑C386410
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA
TK1 / TK / Thymidine Kinase Antibody - Western Blot; Sample: Human Hela cell lysate; Primary Ab: 6µg/ml Mouse Anti-Human TK1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19
TK1 / TK / Thymidine Kinase Antibody (FITC) LS‑C694309
Species: Human, Pig
Applications: Western blot
See all TK1 / TK / Thymidine Kinase Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com