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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TIRC7 / TCIRG1 Antibody LS‑C153628

Catalog Number Size Price
LS-C153628-0.1 0.1 mg (1 mg/ml) $365 
Western blot analysis of TCIRG1 in EL4 cell lysate with TCIRG1 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.

TIRC7 / TCIRG1 Antibody LS‑C153628

Antibody:
Rabbit Polyclonal to Human TIRC7 / TCIRG1
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$365 
LS-C153628-0.1
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human TIRC7 / TCIRG1
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TCIRG1 antibody LS-C153628 is an unconjugated rabbit polyclonal antibody to TCIRG1 (TIRC7 ) from human, mouse and rat. Validated for ELISA and WB.
Target
Human TIRC7 / TCIRG1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • Western blot (0.5 - 1 µg/ml)
  • ELISA
Immunogen
a 19 amino acid synthetic peptide near the N-terminus of human TCIRG1. The immunogen is located within amino acids 70 - 120 of TCIRG1.
Blocking Peptide
LS-E31311 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-C153628. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
At least two alternatively spliced isoforms are known to exist; this antibody will only detect the larger isoform.
Usage
TCIRG1 antibody can be used for detection of TCIRG1 by Western blot at 0.5 ug/mL. Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Western Blot: 91 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TIRC7 / TCIRG1
Q13488 NM_006019 NP_006010.2

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Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
Product
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Anti-TCIRG1 Antibody (C-Term) at 1:2000 dilution + EL4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human, Mouse
Applications: Western blot, Flow Cytometry, ELISA
Product
Species: Human, Mouse, Rat
Applications: Western blot
Reactivity: Human
Range: 15.63-1000 pg/ml
Reactivity: Human
Range: 9.38-600 pg/ml

Requested From: United States
Date Requested: 8/25/2019
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