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Antibodies > THY1 / CD90 > LS-C662121
 
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Catalog Number Size Price
LS-C662121-10 10 µg $318 
LS-C662121-100 100 µg $470 
THY1 / CD90 Antibody - Immunohistochemistry - Anti-CD90/Thy1 Picoband Antibody
THY1 / CD90 Antibody - Immunohistochemistry - Anti-CD90/Thy1 Picoband Antibody
THY1 / CD90 Antibody - Western blot analysis of CD90/Thy1 using anti-CD90/Thy1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD90/Thy1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 26KD. The expected band size for CD90/Thy1 is at 18KD.
THY1 / CD90 Antibody - Western blot - Anti-CD90/Thy1 Picoband Antibody
THY1 / CD90 Antibody - Flow Cytometry analysis of Neuro-2a cells using anti-CD90/Thy1 antibody. Overlay histogram showing Neuro-2a cells stained with anti-CD90/Thy1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD90/Thy1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
THY1 / CD90 Antibody - Flow Cytometry analysis of YAC-1 cells using anti-CD90/Thy1 antibody. Overlay histogram showing YAC-1 cells stained with anti-CD90/Thy1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD90/Thy1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
THY1 / CD90 Antibody - Flow Cytometry analysis of SP2-0 cells using anti-CD90/Thy1 antibody. Overlay histogram showing SP2-0 cells stained with anti-CD90/Thy1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD90/Thy1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
THY1 / CD90 Antibody - Immunohistochemistry - Anti-CD90/Thy1 Picoband Antibody
THY1 / CD90 Antibody - Immunohistochemistry - Anti-CD90/Thy1 Picoband Antibody
THY1 / CD90 Antibody - Western blot analysis of CD90/Thy1 using anti-CD90/Thy1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD90/Thy1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 26KD. The expected band size for CD90/Thy1 is at 18KD.
THY1 / CD90 Antibody - Western blot - Anti-CD90/Thy1 Picoband Antibody
THY1 / CD90 Antibody - Flow Cytometry analysis of Neuro-2a cells using anti-CD90/Thy1 antibody. Overlay histogram showing Neuro-2a cells stained with anti-CD90/Thy1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD90/Thy1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
THY1 / CD90 Antibody - Flow Cytometry analysis of YAC-1 cells using anti-CD90/Thy1 antibody. Overlay histogram showing YAC-1 cells stained with anti-CD90/Thy1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD90/Thy1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
THY1 / CD90 Antibody - Flow Cytometry analysis of SP2-0 cells using anti-CD90/Thy1 antibody. Overlay histogram showing SP2-0 cells stained with anti-CD90/Thy1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD90/Thy1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Mouse THY1 / CD90 Antibody (IHC, WB) LS‑C662121

Polyclonal Rabbit anti‑Mouse THY1 / CD90 Antibody (IHC, WB) LS‑C662121

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Antibody:
THY1 / CD90 Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662121-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
THY1 / CD90 Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD90 antibody LS-C662121 is an unconjugated rabbit polyclonal antibody to CD90 (THY1) from mouse. It is reactive with mouse and rat. Validated for Flow, ICC, IHC and WB.
Target
Mouse THY1 / CD90
Synonyms
THY1 | CD90 antigen | CD90 | CDw90 | Thy-1 antigen | Thy-1 T-cell antigen | Thy-1 | Thy-1 cell surface antigen | Thy-1 membrane glycoprotein
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively.
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About THY1 / CD90
P04216 NM_006288 NP_006279.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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