Human TGFA / TGF Alpha

Mouse

Human, Bovine, Dog, Horse, Rabbit, Sheep, Zebrafish (tested or 100% immunogen sequence identity)

IgM,k Monoclonal

SPM542

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free.

Synthetic peptide (PPVAAAVVSH, aa34-43 of UniProt P01135) from the human protein. Percent identity by BLAST analysis: Human, Ferret, Sheep, Cat, Bovine, Elephant, Rabbit, Horse, Dog (100%); Mouse, Rat, Hamster, Pig, Opossum, Guinea pig, Chicken, Xenopus (90%); Capsaspora, Shewanella (80%).

aa34-43

Human TGFA / TGF Alpha

• IHC
• IHC - Paraffin (2 - 4 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. The optimal dilution of the anti-TGF-alpha antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01135 NCBI: NM_003236 NP_003227.1