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Antibodies > TF / Transferrin > LS-C745251
 
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Catalog Number Size Price
LS-C745251-100 100 µg (1 mg/ml) $544 
TF / Transferrin Antibody - Rockland primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (1° 109-4134, green 2° 611-743-127); Alpha 1 anti trypsin (1° 100-101-147, red 2° 605-742-125); and Human IgG (1° 109-3102, Blue 2° 610-741-124 in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in Blocking Buffer for Fluorescent Western Blotting - MB-070 and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in MB-070 and incubated ~ 1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Biorad VersaDoc4000.
TF / Transferrin Antibody - Rabbit anti-Transferrin (109-4134 lot 3033, green), Goat-anti-Alpha-1-Anti-Trypsin (100-101-147 lot 5842), and Mouse-a-GST (200-301-200 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (611-742-127 lot 21100, shown as green)   DyLight 488 Donkey anti-Mouse IgG (610-741-124 lot 21095, shown as blue),  and DyLight 649 Donkey anti-Goat IgG (605-743-125 lot 20834, shown as red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
TF / Transferrin Antibody - Rockland primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (1° 109-4134, green 2° 611-743-127); Alpha 1 anti trypsin (1° 100-101-147, red 2° 605-742-125); and Human IgG (1° 109-3102, Blue 2° 610-741-124 in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in Blocking Buffer for Fluorescent Western Blotting - MB-070 and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in MB-070 and incubated ~ 1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Biorad VersaDoc4000.
TF / Transferrin Antibody - Rabbit anti-Transferrin (109-4134 lot 3033, green), Goat-anti-Alpha-1-Anti-Trypsin (100-101-147 lot 5842), and Mouse-a-GST (200-301-200 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (611-742-127 lot 21100, shown as green)   DyLight 488 Donkey anti-Mouse IgG (610-741-124 lot 21095, shown as blue),  and DyLight 649 Donkey anti-Goat IgG (605-743-125 lot 20834, shown as red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
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Polyclonal Rabbit anti‑Human TF / Transferrin Antibody (Lyophilized, FITC, IF, WB) LS‑C745251

Polyclonal Rabbit anti‑Human TF / Transferrin Antibody (Lyophilized, FITC, IF, WB) LS‑C745251

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Antibody:
TF / Transferrin Rabbit anti-Human Polyclonal (Lyophilized) (FITC) Antibody
Application:
IF, WB, ELISA, FLISA
Reactivity:
Human
Format:
FITC, Lyophilized
Price
Catalog Number
$544
LS-C745251-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TF / Transferrin Rabbit anti-Human Polyclonal (Lyophilized) (FITC) Antibody
Application:
IF, WB, ELISA, FLISA
Reactivity:
Human
Format:
FITC, Lyophilized

Specifications

Description
Transferrin antibody LS-C745251 is an FITC-conjugated rabbit polyclonal antibody to human Transferrin (TF). Validated for ELISA, FLISA, IF and WB.
Target
Human TF / Transferrin
Synonyms
TF | PRO1557 | Serotransferrin | Siderophilin | TFQTL1 | Beta-1 metal-binding globulin | Transferrin | PRO2086
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
FITC
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Transferrin (Human Serum)
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and purified and partially purified Transferrin (Human Serum).
Applications
  • Immunofluorescence
  • Western blot
  • ELISA (1:10000 - 1:20000)
  • Fluorophore-Linked Immunosorbent Assay
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 10 mg/ml PEG, 0.01% Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TF / Transferrin
P02787 NM_001063 NP_001054.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/14/2024
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