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Antibodies > TF / Transferrin > LS-C745250
 
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Catalog Number Size Price
LS-C745250-25 25 µl (1 mg/ml) $304 
TF / Transferrin Antibody - Immunohistochemistry of rabbit anti human Transferrin antibody. Tissue: Proliferant, cycling human thymus. Antigen Retrieval: HIER pH6.2. Staining: diffuse cytoplasmic and membrane staining. Right: Transferrin antibody 209-4134 at 1:100. Left: Normal Rabbit IgG Negative Control.
TF / Transferrin Antibody - Western Blot of primary and Dylight conjugated secondary antibodies. Lane 1: molecular weight. Lane 2: Rabbit anti-Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Primary antibody: Each primary antibody was diluted to 1:1000 for 2 hrs at RT. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight 488 Conjugated (blue)secondary antibody at 1:10,000 for 45 min at RT. Block: IRdye blocking buffer (MB-070) 1hr at 4°C. Other band(s): none.
TF / Transferrin Antibody - Western Blot of rabbit anti-Transferrin antibody. Lane 1: molecular weight. Lane 2: anti-Transferrin (green), Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Primary antibody: Transferrin antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10,000 for 45 min at RT. Block: 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Other band(s): none.
TF / Transferrin Antibody - Western Blot of rabbit Anti-Transferrin primary antibody. Lane 1: Human Transferrin. Lane 2: None. Primary antibody: Transferrin primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 76-81 kDa. 76 kDa for Transferrin. Other band(s): Transferrin slice varients and isoforms.
TF / Transferrin Antibody - Immunohistochemistry of rabbit anti human Transferrin antibody. Tissue: Proliferant, cycling human thymus. Antigen Retrieval: HIER pH6.2. Staining: diffuse cytoplasmic and membrane staining. Right: Transferrin antibody 209-4134 at 1:100. Left: Normal Rabbit IgG Negative Control.
TF / Transferrin Antibody - Western Blot of primary and Dylight conjugated secondary antibodies. Lane 1: molecular weight. Lane 2: Rabbit anti-Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Primary antibody: Each primary antibody was diluted to 1:1000 for 2 hrs at RT. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight 488 Conjugated (blue)secondary antibody at 1:10,000 for 45 min at RT. Block: IRdye blocking buffer (MB-070) 1hr at 4°C. Other band(s): none.
TF / Transferrin Antibody - Western Blot of rabbit anti-Transferrin antibody. Lane 1: molecular weight. Lane 2: anti-Transferrin (green), Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Primary antibody: Transferrin antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10,000 for 45 min at RT. Block: 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Other band(s): none.
TF / Transferrin Antibody - Western Blot of rabbit Anti-Transferrin primary antibody. Lane 1: Human Transferrin. Lane 2: None. Primary antibody: Transferrin primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 76-81 kDa. 76 kDa for Transferrin. Other band(s): Transferrin slice varients and isoforms.
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Polyclonal Rabbit anti‑Human TF / Transferrin Antibody (Liquid, IHC, WB) LS‑C745250

Polyclonal Rabbit anti‑Human TF / Transferrin Antibody (Liquid, IHC, WB) LS‑C745250

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Antibody:
TF / Transferrin Rabbit anti-Human Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Liquid
Price
Catalog Number
$304
LS-C745250-25
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TF / Transferrin Rabbit anti-Human Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Liquid

Specifications

Description
Transferrin antibody LS-C745250 is an unconjugated rabbit polyclonal antibody to human Transferrin (TF). Validated for ELISA, IHC and WB.
Target
Human TF / Transferrin
Synonyms
TF | PRO1557 | Serotransferrin | Siderophilin | TFQTL1 | Beta-1 metal-binding globulin | Transferrin | PRO2086
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Transferrin Antibody was produced by repeated immunizations with human serum Transferrin protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Transferrin [Human Serum]. Cross reactivity against Transferrin from other tissues and species may occur but have not been specifically determined.
Applications
  • IHC (1:500 - 1:10000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:150000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TF / Transferrin
P02787 NM_001063 NP_001054.1

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TF / Transferrin Antibody - Anti-TF / Transferrin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ TF / Transferrin Antibody LS‑B426
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IHC‑plus™ TF / Transferrin Antibody (clone HTF‑14) LS‑B1856
Species: Pig, Human, Rabbit, Porcine
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See all TF / Transferrin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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