Human TAG-72

Mouse

Human, Rat, Bovine, Dog, Hamster (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

SPM148

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free.

Membrane-enriched fraction of a human breast carcinoma liver metastasis.

Human TAG-72

• IHC
• Immunofluorescence (1 - 2 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Recognizes an oncofetal antigen of 220kDa, identified as a Tumor-associated glycoprotein (TAG-72) with properties of a mucin. This mAb defines the mucin-carried sialylated-Tn epitope. TAG72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma. The optimal dilution of the TAG-72 antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.