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Catalog Number Size Price
LS-C154006-100 100 µg (1 mg/ml) $425 
STAT5A Antibody - Anti-Stat5 pY694 Monoclonal Antibody - Immunohistochemistry. Immunohistochemistry using Anti-Stat5 pY694 monoclonal antibody shows detection of phosphorylated Stat5pY694 in human breast tissue (40X). The antibody was used a dilution to 20 ug/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. Antigen retrieval: steam sections in 0.1 M sodium citrate buffer, pH 6, for 20 min. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
STAT5A Antibody - Anti-Stat5 pY694 Monoclonal Antibody - Immunofluorescent Microscopy. Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody (1:500) detecting Stat5 in 3T3 cells (immunofluorescent STED microscopy). Red represents Stat5 pY694 protein. Green represents tubulin.
STAT5A Antibody - Anti-Stat5 pY694 Monoclonal Antibody - Western Blot. Western blot of Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kD) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution, overnight at 4C. For detection DyLight800 conjugated Gt-a-Mouse IgG was used at a 1:20000 dilution for 30 min at room temperature followed by visualization using a VersaDoc MP 4000 imaging system (Bio-Rad).
STAT5A Antibody - Anti-Stat5 pY694 Monoclonal Antibody - Immunohistochemistry. Immunohistochemistry using Anti-Stat5 pY694 monoclonal antibody shows detection of phosphorylated Stat5pY694 in human breast tissue (40X). The antibody was used a dilution to 20 ug/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. Antigen retrieval: steam sections in 0.1 M sodium citrate buffer, pH 6, for 20 min. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
STAT5A Antibody - Anti-Stat5 pY694 Monoclonal Antibody - Immunofluorescent Microscopy. Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody (1:500) detecting Stat5 in 3T3 cells (immunofluorescent STED microscopy). Red represents Stat5 pY694 protein. Green represents tubulin.
STAT5A Antibody - Anti-Stat5 pY694 Monoclonal Antibody - Western Blot. Western blot of Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kD) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution, overnight at 4C. For detection DyLight800 conjugated Gt-a-Mouse IgG was used at a 1:20000 dilution for 30 min at room temperature followed by visualization using a VersaDoc MP 4000 imaging system (Bio-Rad).
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STAT5A Antibody (phospho‑Tyr694, clone 5F6.F1) LS‑C154006

STAT5A Antibody (phospho‑Tyr694, clone 5F6.F1) LS‑C154006

Antibody:
Mouse Monoclonal to Mouse STAT5A
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154006-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Mouse STAT5A
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
STAT5A antibody LS-C154006 is an unconjugated mouse monoclonal antibody to STAT5A from human, mouse and rat. Validated for ELISA, IF, IHC and WB.
Target
Mouse STAT5A
Host
Mouse
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
5F6.F1
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 - 40 µg/ml)
  • Immunofluorescence (1:50 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
Epitope
pTyr694
Specificity
This antibody is specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.
Usage
Phospho STAT5 pY694 monoclonal antibody is suitable for Immunofluorescence microscopy, ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kD in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About STAT5A
P42229 NM_003152 NP_003143.2

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Requested From: United States
Date Requested: 11/14/2019