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Catalog Number Size Price
LS-C153860-100 100 µg (1 mg/ml) $502 
STAT3 Antibody - Immunohistochemistry of Rabbit anti STAT3 pY705 Antibody. Tissue: Human Colon at 40X in colon tissue at pH 9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: STAT3 pY705 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: STAT3 phospho Y705 is nuclear and cytoplasmic. Staining: STAT3 (pTyr705) as precipitated red signal with hematoxylin purple nuclear counterstain.

Polyclonal Rabbit anti‑Human STAT3 Antibody (phospho‑Tyr705, IHC, WB) LS‑C153860

Polyclonal Rabbit anti‑Human STAT3 Antibody (phospho‑Tyr705, IHC, WB) LS‑C153860

Antibody:
STAT3 Rabbit anti-Human Polyclonal (pTyr705) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$502
LS-C153860-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
STAT3 Rabbit anti-Human Polyclonal (pTyr705) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
STAT3 antibody LS-C153860 is an unconjugated rabbit polyclonal antibody to human STAT3 (pTyr705). Validated for ELISA, IHC and WB.
Target
Human STAT3
Synonyms
STAT3 | Acute-phase response factor | APRF | DNA-binding protein APRF | HIES
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified Stat3 antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids surrounding tyrosine 705 of human Stat3.
Epitope
pTyr705
Specificity
A BLAST analysis was used to suggest cross reactivity with Stat3 antibodies from human, mouse, rat, and pig based on 100%sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Applications
  • IHC
  • Western blot (1 µg/ml)
  • ELISA (1:10000 - 1:30000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 88.1 kD in size corresponding to phosphorylated Stat3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About STAT3
P40763 NM_003150 NP_003141.2

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Requested From: United States
Date Requested: 5/4/2024