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Antibodies > STAT3 > LS-C750557
 
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Catalog Number Size Price
LS-C750557-20 20 µl $287 
LS-C750557-100 100 µl $431 
STAT3 Antibody - Western blot analysis of extracts of various cell lines, using Phospho-Stat3-S727 antibody at 1:1000 dilution or STAT3 antibody. NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 30 minutes or treated by UV at room temperature for 15-30 minutes. C6 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 20 minutes or treated by UV at room temperature for 15-30 minutes. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.
STAT3 Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-Stat3-S727 pAb. Western blot was performed from the immunoprecipitate using Phospho-Stat3-S727 pAb at a dilition of 1:1000. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight.
STAT3 Antibody - Western blot analysis of extracts of various cell lines, using Phospho-Stat3-S727 antibody at 1:1000 dilution or STAT3 antibody. NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 30 minutes or treated by UV at room temperature for 15-30 minutes. C6 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 20 minutes or treated by UV at room temperature for 15-30 minutes. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.
STAT3 Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-Stat3-S727 pAb. Western blot was performed from the immunoprecipitate using Phospho-Stat3-S727 pAb at a dilition of 1:1000. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight.
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Polyclonal Rabbit anti‑Human STAT3 Antibody (phospho‑Ser727, WB) LS‑C750557

Polyclonal Rabbit anti‑Human STAT3 Antibody (phospho‑Ser727, WB) LS‑C750557

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Antibody:
STAT3 Rabbit anti-Human Polyclonal (pSer727) Antibody
Application:
WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C750557-20
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
STAT3 Rabbit anti-Human Polyclonal (pSer727) Antibody
Application:
WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
STAT3 antibody LS-C750557 is an unconjugated rabbit polyclonal antibody to STAT3 (pSer727) from human. It is reactive with human, mouse and rat. Validated for IP and WB.
Target
Human STAT3
Synonyms
STAT3 | Acute-phase response factor | APRF | DNA-binding protein APRF | HIES
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Epitope
pSer727
Applications
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:100)
Usage
The predicted MW is 83kDa/87kDa/88kDa, while the observed MW by Western blot was 86kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About STAT3
P40763 NM_003150 NP_003141.2

Publications (0)

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STAT3 Antibody - Immunohistochemical analysis of STAT3 (pY705) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
STAT3 Antibody (phospho‑Tyr705) LS‑C354108
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/6/2024
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