Human SPTBN2

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Recombinant Monoclonal

SPTBN2/2894R

Unconjugated

Protein A/G purified

Unmodified. Also available Azide-free, Carrier-free.

Recombinant human SPTBN2 fragment (aa356-475). Antigen Molecular Weight: 246 kDa

aa356-475

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alpha II (also designated fodrin alpha) and spectrin beta I (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin beta III is highly homologous to both spectrin beta I and spectrin beta II. Spectrin beta III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

• IHC - Paraffin (0.5 - 1 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Flow Cytometry (1 - 2 µg/10E6 cells)
• ELISA (2 - 4 µg/ml)

Western Blotting (0.5-1.0 ug/ml) - Immunohistology (Formalin-fixed - 0.5-1.0 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS, 0.05% BSA, 0.05% Sodium Azide

Store at 2°C to 8°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O15020 NCBI: NM_006946 NP_008877.1