Recombinant Monoclonal Rabbit anti‑Human SPN / CD43 Antibody (clone SPN/1766R, Azide‑free,Carrier‑free, IHC, IF, WB) LS‑C751369
Recombinant Monoclonal Rabbit anti‑Human SPN / CD43 Antibody (clone SPN/1766R, Azide‑free,Carrier‑free, IHC, IF, WB) LS‑C751369
Antibody:
SPN / CD43 Rabbit anti-Human Recombinant Monoclonal (Azide-free, Carrier-free) (SPN/1766R) Antibody
Application:
IHC-P, IF, WB, Flo
Format:
Unconjugated, Azide-free, Carrier-free
Overview
Antibody:
SPN / CD43 Rabbit anti-Human Recombinant Monoclonal (Azide-free, Carrier-free) (SPN/1766R) Antibody
Application:
IHC-P, IF, WB, Flo
Format:
Unconjugated, Azide-free, Carrier-free
Specifications
Description
CD43 antibody LS-C751369 is an unconjugated rabbit recombinant monoclonal antibody to human CD43 (SPN). Validated for Flow, IF, IHC and WB.
Target
Human SPN / CD43
Synonyms
SPN | GPL115 | Leukosialin | Leukocyte sialoglycoprotein | Galactoglycoprotein | GALGP | Sialophorin | LSN | CD43 | CD43 antigen
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Recombinant Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Azide-free, Carrier-free.
Also available
Unmodified.
Immunogen
Recombinant full-length human SPN protein. Antigen Molecular Weight: 95, 115, or 135 kDa
Specificity
It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Applications
- IHC - Paraffin (0.5 - 1 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Storage
Store at -20°C to -80°C for up to 2 years.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
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Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/13/2024