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Catalog Number Size Price
LS-C782440-20 20 µg (0.2 mg/ml) $371 
LS-C782440-100 100 µg (0.2 mg/ml) $574 
SPI1 / PU.1 Antibody - IHC staining of FFPE Hodgkins lymphoma with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SPI1 / PU.1 Antibody - IHC staining of FFPE human colon carcinoma with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SPI1 / PU.1 Antibody - IHC staining of FFPE human spleen with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SPI1 / PU.1 Antibody - IHC staining of FFPE human lymph node with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SPI1 / PU.1 Antibody - Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PU.1 antibody (clone PU1/2146). These results demonstrate the foremost specificity of the PU1/2146 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SPI1 / PU.1 Antibody - Western blot testing of human THP-1 cell lysate with PU.1 antibody (clone PU1/2146). Predicted molecular weight ~31 kDa but routinely observed at ~40 kDa.
SPI1 / PU.1 Antibody - SDS-PAGE analysis of purified, BSA-free PU.1 antibody (clone PU1/2146) as confirmation of integrity and purity.
SPI1 / PU.1 Antibody - IHC staining of FFPE Hodgkins lymphoma with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SPI1 / PU.1 Antibody - IHC staining of FFPE human colon carcinoma with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SPI1 / PU.1 Antibody - IHC staining of FFPE human spleen with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SPI1 / PU.1 Antibody - IHC staining of FFPE human lymph node with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SPI1 / PU.1 Antibody - Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PU.1 antibody (clone PU1/2146). These results demonstrate the foremost specificity of the PU1/2146 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SPI1 / PU.1 Antibody - Western blot testing of human THP-1 cell lysate with PU.1 antibody (clone PU1/2146). Predicted molecular weight ~31 kDa but routinely observed at ~40 kDa.
SPI1 / PU.1 Antibody - SDS-PAGE analysis of purified, BSA-free PU.1 antibody (clone PU1/2146) as confirmation of integrity and purity.
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Monoclonal Mouse anti‑Human SPI1 / PU.1 Antibody (clone PU1/2146, IHC, WB) LS‑C782440

Monoclonal Mouse anti‑Human SPI1 / PU.1 Antibody (clone PU1/2146, IHC, WB) LS‑C782440

Antibody:
SPI1 / PU.1 Mouse anti-Human Monoclonal (PU1/2146) Antibody
Application:
IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$371
LS-C782440-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SPI1 / PU.1 Mouse anti-Human Monoclonal (PU1/2146) Antibody
Application:
IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PU.1 antibody LS-C782440 is an unconjugated mouse monoclonal antibody to human PU.1 (SPI1). Validated for IHC and WB.
Target
Human SPI1 / PU.1
Synonyms
SPI1 | 31 kDa-transforming protein | 31 kDa transforming protein | PU.1 | SFPI1 | SPI-1 | SPI-1 proto-oncogene | SPI-A | Transcription factor PU.1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
PU1/2146
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
A portion of amino acids 16-170 from the human protein was used as the immunogen for the PU.1 antibody.
Specificity
This antibody has been extensively validated, including cross-reactivity testing against 19,000 full-length proteins by HuProt(TM) microarray analysis.
Applications
  • IHC - Paraffin (1 - 2 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Optimal dilution of the PU.1 antibody should be determined by the researcher.
Presentation
1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage
Store at 2-8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPI1 / PU.1
P17947 NM_003120 NP_003111.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024