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Catalog Number Size Price
LS-C358335-100 100 µl (1 mg/ml) $305 
SP1 Antibody - Immunohistochemical analysis of SP1 (pT453) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
SP1 Antibody - Immunofluorescent analysis of SP1 (pT453) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
SP1 Antibody - Western blot analysis of SP1 (pT453) expression in Jurkat (A); mouse melanoma cancer (B) whole cell lysates.
SP1 Antibody - Immunohistochemical analysis of SP1 (pT453) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
SP1 Antibody - Immunofluorescent analysis of SP1 (pT453) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
SP1 Antibody - Western blot analysis of SP1 (pT453) expression in Jurkat (A); mouse melanoma cancer (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human SP1 Antibody (phospho‑Thr453, IHC, IF, WB) LS‑C358335

Polyclonal Rabbit anti‑Human SP1 Antibody (phospho‑Thr453, IHC, IF, WB) LS‑C358335

Antibody:
SP1 Rabbit anti-Human Polyclonal (pThr453) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$305
LS-C358335-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
SP1 Rabbit anti-Human Polyclonal (pThr453) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
SP1 antibody LS-C358335 is an unconjugated rabbit polyclonal antibody to SP1 (pThr453) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human SP1
Synonyms
SP1 | Sp1 transcription factor | Transcription factor Sp1 | Specificity protein 1 | TSFP1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human SP1 (pT453).
Epitope
pThr453
Specificity
Recognizes endogenous levels of SP1 (pT453) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SP1
P08047 NM_138473 NP_612482.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/9/2021