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Catalog Number	Size	Price
LS-C748405-20	20 µl	$287
LS-C748405-100	100 µl	$431

SMARCE1 / BAF57 Antibody - Immunofluorescence analysis of HeLa cells using SMARCE1 antibody.

SMARCE1 / BAF57 Antibody - Western blot analysis of extracts from normal (control) and SMARCE1 knockout (KO) HeLa cells, using SMARCE1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 15s.

SMARCE1 / BAF57 Antibody - Western blot analysis of extracts of various cell lines, using SMARCE1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

SMARCE1 / BAF57 Antibody - Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SMARCE1 antibody. Western blot was performed from the immunoprecipitate using SMARCE1 antibody at a dilition of 1:1000.

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Polyclonal Rabbit anti‑Human SMARCE1 / BAF57 Antibody (IHC, IF, WB) LS‑C748405

Name: Anti-SMARCE1 / BAF57 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C748405-20
Price: 287.0000 USD
Availability: InStock

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Antibody:

SMARCE1 / BAF57 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, IP

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$287

LS-C748405-20

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

SMARCE1 / BAF57 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, IP

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

BAF57 antibody LS-C748405 is an unconjugated rabbit polyclonal antibody to BAF57 (SMARCE1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP and WB.

Target

Human SMARCE1 / BAF57

Synonyms

SMARCE1 | BRG1-associated factor 57 | BAF57

See All SMARCE1 / BAF57 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purified

Modifications

Unmodified

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3). MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSRVTASSGITIPKPPKPPDKPLMPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQEYLNEYEAEKIEYNESMKAYHNSPAYLAYINAKSRAEAALEEESRQRQSRMEKGEPYMSIQPAEDPDDYDDGFSMKHTATARFQRNHRLISEILSESVVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEERHQEKKRKFLESTDSFNNELKRLCGLKVEVDMEKIAAEIAQAEEQARKRQEEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSD

Applications

IHC (1:50 - 1:200)
Immunofluorescence (1:50 - 1:200)
Western blot (1:500 - 1:2000)
Immunoprecipitation (1:20 - 1:50)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

The predicted MW is 34kDa/37kDa/39kDa/41kDa/42kDa/46kDa, while the observed MW by Western blot was 55kDa.

Presentation

PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About SMARCE1 / BAF57