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Catalog Number Size Price
LS-C22950-50 50 µg $745 
Antibody

Polyclonal Rabbit anti‑Rat SLC6A3 / Dopamine Transporter Antibody (N‑Terminus, IHC, WB) LS‑C22950

Polyclonal Rabbit anti‑Rat SLC6A3 / Dopamine Transporter Antibody (N‑Terminus, IHC, WB) LS‑C22950

Antibody:
SLC6A3 / Dopamine Transporter Rabbit anti-Rat Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-Fr, WB, IP, ELISA
Reactivity:
Rat, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$745
LS-C22950-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SLC6A3 / Dopamine Transporter Rabbit anti-Rat Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-Fr, WB, IP, ELISA
Reactivity:
Rat, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Dopamine Transporter antibody LS-C22950 is an unconjugated rabbit polyclonal antibody to Dopamine Transporter (SLC6A3) (N-Terminus) from rat. It is reactive with mouse and rat. Validated for ELISA, IHC, IP and WB.
Target
Rat SLC6A3 / Dopamine Transporter
Synonyms
SLC6A3 | DA transporter | DAT1 | DAT
Host
Rabbit
Reactivity
Rat, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (1). This region is presumably the cytoplasmic domain.
Epitope
N-Terminus
Specificity
Recognizes Dopamine transporter (DAT). Species cross-reactivity: Rat and mouse. Weakly reacts with human and canine.
Applications
  • IHC
  • IHC - Frozen
  • Western blot (1 - 10 µg/ml)
  • Immunoprecipitation
  • ELISA (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and ELISA. Western Blot: 1-10 ug/ml using ECL. An antibody raised against a similar peptide has been shown to react with a broad 80kD band in rat striatum and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55kD. Immunoprecipitation: 2-10 ug. Immunohistochemistry: 2-10 ug/ml on frozen sections of tissues fixed with 4% paraformaldehyde. ELISA: 0.5-1.0 ug/ml using 1 ug/ml of DAT control peptide per well.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide, 0.1% BSA
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC6A3 / Dopamine Transporter
Q01959 NM_001044 NP_001035.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022