Human SLC6A1 / GAT-1

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-EQPQAGSSTSKE, from the C-Terminus of protein sequence according to NP_003033.3.

aa585-596

Human SLC6A1 / GAT-1

• Peptide Enzyme-Linked Immunosorbent Assay (1:4000)

Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments gave bands at approx 50kD and 37kD in Human Brain (Cerebellum) lysates after 2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 67.1kD according to NP_003033.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P30531 NCBI: NM_003042 NP_003033.3