Recognizes rat Glut-5. Species sequence homology: human: 61%, mouse: 53%.
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Western Blot: 1-10 ug/ml using ECL. The antibody has been shown to react with a protein band of about 60kD in the rat jejunum membranes that can be competed with the control peptide. The MW Of Glut 5 is larger than that reported for human Glut 5 (50-55kD). As opposed to human tissues, rat Glut-5 mRNA has not been detected in rat testis, adipose tissues, and skeletal muscle. In rat kidney, a protein of about 42kD was detected. Differences in MW of Glut-3 in various tissues has also been noted. ELISA: 1:1000-1:10000. Use control peptide to coat ELISA plates at 1 ug/ml/.
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.