GLUT5 antibody LS-C15414 is an unconjugated rabbit polyclonal antibody to rat GLUT5 (SLC2A5). Validated for ELISA, IF, IHC and WB. Cited in 2 publications.
Rat SLC2A5 / GLUT5
(tested or 100% immunogen sequence identity)
- Western blot (1 - 10 µg/ml)
- ELISA (1:1000 - 1:10000)
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SLC2A5 / GLUT5 antibody was raised against a cytoplasmic 13 AA peptide sequence (aa 490-502) near the C-terminus of rat liver glucose/fructose transporter (Glut-5) (1). A cysteine has been added at the NH2 terminus for coupling to KLH. The peptide was coupled to KLH.
Recognizes rat Glut-5. Species sequence homology: human: 61%, mouse: 53%.
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Western Blot: 1-10 ug/ml using ECL. The antibody has been shown to react with a protein band of about 60kD in the rat jejunum membranes that can be competed with the control peptide. The MW Of Glut 5 is larger than that reported for human Glut 5 (50-55kD). As opposed to human tissues, rat Glut-5 mRNA has not been detected in rat testis, adipose tissues, and skeletal muscle. In rat kidney, a protein of about 42kD was detected. Differences in MW of Glut-3 in various tissues has also been noted. ELISA: 1:1000-1:10000. Use control peptide to coat ELISA plates at 1 ug/ml/.
PBS, pH 7.4, 0.1% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.