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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SLC29A1 / ENT1 Antibody (aa234‑263) IHC‑plus™ LS‑B3385 Best Seller
Note: This antibody replaces LS-C96640
ENT1 antibody LS-B3385 is an unconjugated rabbit polyclonal antibody to ENT1 (SLC29A1) from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Catalog
Size
Price
LS-B3385-200
200 µl
$425
Description
ENT1 antibody LS-B3385 is an unconjugated rabbit polyclonal antibody to ENT1 (SLC29A1) from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Rat SLC29A1 / ENT1
Host
Rabbit
Reactivity
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E566 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3385. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa234-263
Specificity
This ENT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-263 amino acids from the Central region of rat ENT1(O54698).
Usage
Immunohistochemistry: LS-B3385 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3385 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About SLC29A1 / ENT1
Q99808 NM_004955 NP_004946.1

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Anti-ENT1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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Anti-ENT1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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Publications (2)

1
Levels of human equilibrative nucleoside transporter-1 are higher in proliferating regions of A549 tumor cells grown as tumor xenografts in vivo. Plotnik DA, Asher C, Chu SK, Miyaoka RS, Garwin GG, Johnson BW, Li T, Krohn KA, Schwartz JL. Nuclear medicine and biology. 2012 39:1161-6. (IHC; Human) [Full Text Article] [PubMed:22985987] [PMC:PMC3478396]
2
Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer. Zheng X, Carstens JL, Kim J, Scheible M, Kaye J, Sugimoto H, Wu CC, LeBleu VS, Kalluri R. Nature. 2015 527:525-530. (IHC; Mouse) [Full Text Article] [PubMed:26560028] [PMC:PMC4849281]

Customer Reviews (0)


Images

Immunohistochemistry

Anti-ENT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-ENT1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENT1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

ENT1(Slc29a1) western blot of mouse brain tissue lysates (35 ug/lane). The ENT1 antibody detected ENT1 protein (arrow).
ENT1(Slc29a1) western blot of mouse brain tissue lysates (35 ug/lane). The ENT1 antibody detected ENT1 protein (arrow).

Western blot

Western blot of lysate from HL-60 cell line, using ENT1(Slc29a1) Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of lysate from HL-60 cell line, using ENT1(Slc29a1) Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot

Western blot of lysates from human kidney, plasma, mouse kidney, plasma and rat kidney tissue lysate(from left to right), using GPX3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of lysates from human kidney, plasma, mouse kidney, plasma and rat kidney tissue lysate(from left to right), using GPX3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Immunohistochemistry

Anti-ENT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-ENT1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENT1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

ENT1(Slc29a1) western blot of mouse brain tissue lysates (35 ug/lane). The ENT1 antibody detected ENT1 protein (arrow).
ENT1(Slc29a1) western blot of mouse brain tissue lysates (35 ug/lane). The ENT1 antibody detected ENT1 protein (arrow).

Western blot

Western blot of lysate from HL-60 cell line, using ENT1(Slc29a1) Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of lysate from HL-60 cell line, using ENT1(Slc29a1) Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot

Western blot of lysates from human kidney, plasma, mouse kidney, plasma and rat kidney tissue lysate(from left to right), using GPX3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of lysates from human kidney, plasma, mouse kidney, plasma and rat kidney tissue lysate(from left to right), using GPX3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Immunohistochemistry

Anti-ENT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-ENT1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENT1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

ENT1(Slc29a1) western blot of mouse brain tissue lysates (35 ug/lane). The ENT1 antibody detected ENT1 protein (arrow).
ENT1(Slc29a1) western blot of mouse brain tissue lysates (35 ug/lane). The ENT1 antibody detected ENT1 protein (arrow).

Western blot

Western blot of lysate from HL-60 cell line, using ENT1(Slc29a1) Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of lysate from HL-60 cell line, using ENT1(Slc29a1) Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot

Western blot of lysates from human kidney, plasma, mouse kidney, plasma and rat kidney tissue lysate(from left to right), using GPX3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of lysates from human kidney, plasma, mouse kidney, plasma and rat kidney tissue lysate(from left to right), using GPX3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Immunohistochemistry

Anti-ENT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-ENT1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENT1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

ENT1(Slc29a1) western blot of mouse brain tissue lysates (35 ug/lane). The ENT1 antibody detected ENT1 protein (arrow).
ENT1(Slc29a1) western blot of mouse brain tissue lysates (35 ug/lane). The ENT1 antibody detected ENT1 protein (arrow).

Western blot

Western blot of lysate from HL-60 cell line, using ENT1(Slc29a1) Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of lysate from HL-60 cell line, using ENT1(Slc29a1) Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot

Western blot of lysates from human kidney, plasma, mouse kidney, plasma and rat kidney tissue lysate(from left to right), using GPX3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of lysates from human kidney, plasma, mouse kidney, plasma and rat kidney tissue lysate(from left to right), using GPX3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Requested From: United States
Date Requested: 12/14/2018
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