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Catalog Number Size Price
LS-C662248-10 10 µg $318 
LS-C662248-100 100 µg $470 
SIX6 Antibody - Western blot analysis of SIX6 using anti-SIX6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIX6 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SIX6 at approximately 28KD. The expected band size for SIX6 is at 28KD.

Polyclonal Rabbit anti‑Human SIX6 Antibody (WB) LS‑C662248

Polyclonal Rabbit anti‑Human SIX6 Antibody (WB) LS‑C662248

Antibody:
SIX6 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662248-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SIX6 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SIX6 antibody LS-C662248 is an unconjugated rabbit polyclonal antibody to human SIX6. Validated for WB.
Target
Human SIX6
Synonyms
SIX6 | Homeodomain protein OPTX2 | MCOPCT2 | Homeobox protein SIX6 | Six9 | Sine oculis homeobox homolog 6 | SIX homeobox 6 | Optic homeobox 2 | OPTX2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human SIX6 recombinant protein (Position: A188-I246). Human SIX6 shares 91.5% amino acid (aa) sequence identity with mouse SIX6.
Specificity
Expressed in the developing and adult retina. Also expressed in the hypothalamic and the pituitary regions.
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SIX6
O95475 NM_007374 NP_031400.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024