CIN85 antibody LS-C17823 is an unconjugated mouse monoclonal antibody to CIN85 (SH3KBP1) from human and rat. Validated for ICC, IP and WB.
Rat SH3KBP1 / CIN85
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (1 - 2 µg/ml)
- (applications tested for the base form of this product only)
SH3KBP1 / CIN85 antibody was raised against 6His and HA tagged fusion protein corresponding to full length rat SETA.
=Recognizes rat SETA, Mr ~85kD. SETA has multiple splice variants which can result in multiple bands when immunoblotting. Species cross-reactivity: human, mouse.
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1-2 ug/ml detects SETA in RIPA lysates from NG108-15 cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes. Immunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.