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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SH3KBP1 / CIN85 Antibody LS-C17823

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CIN85 antibody LS-C17823 is an unconjugated mouse monoclonal antibody to CIN85 (SH3KBP1) from human and rat. Validated for ICC, IP and WB.
Catalog
Size
Price
LS-C17823-200
200 µg
$695
Description
CIN85 antibody LS-C17823 is an unconjugated mouse monoclonal antibody to CIN85 (SH3KBP1) from human and rat. Validated for ICC, IP and WB.
Target
Rat SH3KBP1 / CIN85
Host
Mouse
Reactivity
Rat, Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot (1 - 2 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
SH3KBP1 / CIN85 antibody was raised against 6His and HA tagged fusion protein corresponding to full length rat SETA.
Specificity
=Recognizes rat SETA, Mr ~85kD. SETA has multiple splice variants which can result in multiple bands when immunoblotting. Species cross-reactivity: human, mouse.
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1-2 ug/ml detects SETA in RIPA lysates from NG108-15 cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes. Immunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SH3KBP1 / CIN85
Q96B97 NM_031892 NP_114098.1

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Requested From: United States
Date Requested: 4/21/2019
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