Target
Rat SH3KBP1 / CIN85
Synonyms
SH3KBP1 | CD2BP3 | CIN85 | GIG10 | HSB-1 | HSB1 | C-Cbl-interacting protein | CD2-binding protein 3 | MIG18 | Migration-inducing gene 18
Reactivity
Rat, Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
6His and HA tagged fusion protein corresponding to full length rat SETA.
Specificity
=Recognizes rat SETA, Mr ~85kD. SETA has multiple splice variants which can result in multiple bands when immunoblotting. Species cross-reactivity: human, mouse.
Applications
- ICC
- Western blot (1 - 2 µg/ml)
- Immunoprecipitation
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1-2 ug/ml detects SETA in RIPA lysates from NG108-15 cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes. Immunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee