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SFTPA1 / Surfactant Protein A Antibody (aa233‑248) LS‑C312616

Catalog Number Size Price
LS-C312616-100 100 µg (0.5 mg/ml) $355 
IHC analysis of SFTPA1 using anti-SFTPA1 antibody. SFTPA1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SFTPA1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SFTPA1 / Surfactant Protein A antibody. IHC(P): Rat Lung Tissue.
WB of SFTPA1 / Surfactant Protein A antibody. Lane 1: Mouse Lung Tissue Lysate. Lane 2: Mouse Lung Tissue Lysate. Lane 3: Rat Lung Tissue Lysate. Lane 4: Rat Lung Tissue Lysate.
Western blot analysis of SFTPA1 using anti-SFTPA1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26KD. The expected band size for SFTPA1 is at 26KD.
IHC analysis of SFTPA1 using anti-SFTPA1 antibody. SFTPA1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SFTPA1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SFTPA1 / Surfactant Protein A antibody. IHC(P): Rat Lung Tissue.
WB of SFTPA1 / Surfactant Protein A antibody. Lane 1: Mouse Lung Tissue Lysate. Lane 2: Mouse Lung Tissue Lysate. Lane 3: Rat Lung Tissue Lysate. Lane 4: Rat Lung Tissue Lysate.
Western blot analysis of SFTPA1 using anti-SFTPA1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26KD. The expected band size for SFTPA1 is at 26KD.
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SFTPA1 / Surfactant Protein A Antibody (aa233‑248) LS‑C312616

Note: This antibody replaces LS-C389289
Antibody:
Rabbit Polyclonal to Mouse SFTPA1 / Surfactant Protein A
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$355 
LS-C312616-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse SFTPA1 / Surfactant Protein A
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Surfactant Protein A antibody LS-C312616 is an unconjugated rabbit polyclonal antibody to Surfactant Protein A (SFTPA1 ) from mouse and rat. Validated for IHC and WB. Cited in 1 publication.
Target
Mouse SFTPA1 / Surfactant Protein A
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse SFTPA1 (233-248 aa WNDKGCLQYRLAICEF), identical to the related rat sequence.
Epitope
aa233-248
Presentation
Contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2 HPO4, 0.05 mg Thimerosal, 0.05 mg sodium azide per 100 ug antibody.
Reconstitution
200 µl Distilled water
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SFTPA1 / Surfactant Protein A
Q8IWL2 NM_005411 NP_005402.3

Publications (1)

Disruption of the structural and functional features of surfactant protein A by acrolein in cigarette smoke. Takamiya R, Uchida K, Shibata T, Maeno T, Kato M, Yamaguchi Y, Ariki S, Hasegawa Y, Saito A, Miwa S, Takahashi H, Akaike T, Kuroki Y, Takahashi M. Scientific reports. 2017 7:8304. (WB; Mouse)

Reviews (0)

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Requested From: United States
Date Requested: 9/18/2019