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Catalog Number Size Price
LS-C312616-10 10 µg $318 
LS-C312616-100 100 µg (0.5 mg/ml) $470 
SFTPA1 / Surfactant Protein A Antibody - IHC analysis of SFTPA1 using anti-SFTPA1 antibody. SFTPA1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SFTPA1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SFTPA1 / Surfactant Protein A Antibody - SFTPA1 / Surfactant Protein A antibody. IHC(P): Rat Lung Tissue.
SFTPA1 / Surfactant Protein A Antibody - Western blot analysis of SFTPA1 using anti-SFTPA1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26KD. The expected band size for SFTPA1 is at 26KD.
SFTPA1 / Surfactant Protein A Antibody - WB of SFTPA1 / Surfactant Protein A antibody. Lane 1: Mouse Lung Tissue Lysate. Lane 2: Mouse Lung Tissue Lysate. Lane 3: Rat Lung Tissue Lysate. Lane 4: Rat Lung Tissue Lysate.
SFTPA1 / Surfactant Protein A Antibody - IHC analysis of SFTPA1 using anti-SFTPA1 antibody. SFTPA1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SFTPA1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SFTPA1 / Surfactant Protein A Antibody - SFTPA1 / Surfactant Protein A antibody. IHC(P): Rat Lung Tissue.
SFTPA1 / Surfactant Protein A Antibody - Western blot analysis of SFTPA1 using anti-SFTPA1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26KD. The expected band size for SFTPA1 is at 26KD.
SFTPA1 / Surfactant Protein A Antibody - WB of SFTPA1 / Surfactant Protein A antibody. Lane 1: Mouse Lung Tissue Lysate. Lane 2: Mouse Lung Tissue Lysate. Lane 3: Rat Lung Tissue Lysate. Lane 4: Rat Lung Tissue Lysate.
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Polyclonal Rabbit anti‑Mouse SFTPA1 / Surfactant Protein A Antibody (aa233‑248, IHC, WB) LS‑C312616

Polyclonal Rabbit anti‑Mouse SFTPA1 / Surfactant Protein A Antibody (aa233‑248, IHC, WB) LS‑C312616

Note: This antibody replaces LS-C389289
Antibody:
SFTPA1 / Surfactant Protein A Rabbit anti-Mouse Polyclonal (aa233-248) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312616-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SFTPA1 / Surfactant Protein A Rabbit anti-Mouse Polyclonal (aa233-248) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Surfactant Protein A antibody LS-C312616 is an unconjugated rabbit polyclonal antibody to Surfactant Protein A (SFTPA1) (aa233-248) from mouse. It is reactive with mouse and rat. Validated for IHC and WB. Cited in 1 publication.
Target
Mouse SFTPA1 / Surfactant Protein A
Synonyms
SFTPA1 | Alveolar proteinosis protein | COLEC4 | Collectin-4 | PSP-A | PSPA | SFTP1 | SFTPA | SFTPA1B | SPA1 | SP-A | SP-A1 | SPA | Surfactant Protein A | Surfactant protein A1 | Surfactant protein A1B | Surfactant A
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of mouse SFTPA1 (233-248 aa WNDKGCLQYRLAICEF), identical to the related rat sequence.
Epitope
aa233-248
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SFTPA1 / Surfactant Protein A
Q8IWL2 NM_005411 NP_005402.3

LSBio Ratings

SFTPA1 / Surfactant Protein A Antibody (aa233-248) for IHC, WB/Western LS-C312616 has an LSBio Rating of
Publications (4)

Publications (1)

Disruption of the structural and functional features of surfactant protein A by acrolein in cigarette smoke. Takamiya R, Uchida K, Shibata T, Maeno T, Kato M, Yamaguchi Y, Ariki S, Hasegawa Y, Saito A, Miwa S, Takahashi H, Akaike T, Kuroki Y, Takahashi M. Scientific reports. 2017 7:8304. (WB; Mouse)

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Requested From: United States
Date Requested: 5/22/2024