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Catalog Number Size Price
LS-C818411-100 100 µl $412 
SFN / Stratifin / 14-3-3 Sigma Antibody - Immunochemical staining of mouse SFN in mouse skin with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.
SFN / Stratifin / 14-3-3 Sigma Antibody - Immunofluorescence staining of mSFN in Raw264.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse mSFN polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.
SFN / Stratifin / 14-3-3 Sigma Antibody - Anti-SFN rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH3T3 Whole Cell Lysate. Lane B: Raw 264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa.
SFN / Stratifin / 14-3-3 Sigma Antibody - Mouse SFN was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. 4 uL anti-Mouse SFN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Mouse SFN rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa.
SFN / Stratifin / 14-3-3 Sigma Antibody - Immunochemical staining of mouse SFN in mouse skin with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.
SFN / Stratifin / 14-3-3 Sigma Antibody - Immunofluorescence staining of mSFN in Raw264.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse mSFN polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.
SFN / Stratifin / 14-3-3 Sigma Antibody - Anti-SFN rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH3T3 Whole Cell Lysate. Lane B: Raw 264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa.
SFN / Stratifin / 14-3-3 Sigma Antibody - Mouse SFN was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. 4 uL anti-Mouse SFN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Mouse SFN rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa.
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Polyclonal Rabbit anti‑Mouse SFN / Stratifin / 14‑3‑3 Sigma Antibody (Preservative Free, IHC, IF, WB) LS‑C818411

Polyclonal Rabbit anti‑Mouse SFN / Stratifin / 14‑3‑3 Sigma Antibody (Preservative Free, IHC, IF, WB) LS‑C818411

Antibody:
SFN / Stratifin / 14-3-3 Sigma Rabbit anti-Mouse Polyclonal (Preservative Free) Antibody
Application:
IHC-P, ICC, IF, WB, IP, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C818411-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SFN / Stratifin / 14-3-3 Sigma Rabbit anti-Mouse Polyclonal (Preservative Free) Antibody
Application:
IHC-P, ICC, IF, WB, IP, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Preservative Free

Specifications

Description
14-3-3 Sigma antibody LS-C818411 is an unconjugated rabbit polyclonal antibody to mouse 14-3-3 Sigma (SFN / Stratifin). Validated for ELISA, ICC, IF, IHC, IP and WB.
Target
Mouse SFN / Stratifin / 14-3-3 Sigma
Synonyms
SFN | 14-3-3 sigma | 14-3-3 protein sigma | HME1 | Stratifin | YWHAS
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
Recombinant Mouse 14-3-3 sigma/SFN Protein
Specificity
Mouse 14-3-3 sigma
Applications
  • IHC - Paraffin (1:2000 - 1:20000)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
  • ELISA (1:5000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SFN / Stratifin / 14-3-3 Sigma
P31947 NM_006142 NP_006133.1

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Requested From: United States
Date Requested: 4/26/2024