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Antibodies > SF3B2 > LS-C817211
 
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Catalog Number Size Price
LS-C817211-50 50 µl $317 
LS-C817211-100 100 µl $375 
LS-C817211-200 200 µl $473 
SF3B2 Antibody - Immunofluorescence staining of SF3B2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SF3B2 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
SF3B2 Antibody - Anti-SF3B2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 140 kDa.
SF3B2 Antibody - SF3B2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. 4 uL anti-SF3B2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SF3B2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 150 kDa.
SF3B2 Antibody - Immunofluorescence staining of SF3B2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SF3B2 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
SF3B2 Antibody - Anti-SF3B2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 140 kDa.
SF3B2 Antibody - SF3B2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. 4 uL anti-SF3B2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SF3B2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 150 kDa.
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Polyclonal Rabbit anti‑Human SF3B2 Antibody (IF, WB) LS‑C817211

Polyclonal Rabbit anti‑Human SF3B2 Antibody (IF, WB) LS‑C817211

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Antibody:
SF3B2 Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$317
LS-C817211-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SF3B2 Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SF3B2 antibody LS-C817211 is an unconjugated rabbit polyclonal antibody to human SF3B2. Validated for ICC, IF, IP and WB.
Target
Human SF3B2
Synonyms
SF3B2 | Cus1 | SAP 145 | SAP145 | SF3B145 | Splicing factor 3B subunit 2 | SF3b150
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
E. coli-derived Human SF3B2 fragment
Specificity
Human SF3B2
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.0, 0.03% Proclin 300
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SF3B2
Q13435 BC000401 AAH00401.2

Publications (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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