Mouse Serum Proteins

Rabbit

Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Delipidated and heat inactivated

Unmodified

Pooled immunoglobulin-depleted mouse serum and partly purified serum fractions. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Precipitating polyclonal Rabbit antiserum to Mouse Serum proteins without immunoglobulins. Inter-species cross reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross-reactivity. Consequently it is not species-specific.

• Immunoelectrophoresis

In immunoelectrophoresis use 2 µl serum or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual serum protein components.

Lyophilized from stable whole antiserum

Reconstitute by adding 1 ml sterile distilled water.

Store at 4°C. Aliquot and store at -20°C for long term storage. Avoid freeze/thaw cycles. Prepare working dilutions by adding phosphate buffered saline (PBS, pH 7.2). Slight precipitation can be removed by centrifugation. Store working dilutions at 4°C, and preferably use the same day. Remove slight precipitation with centrifugation.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.