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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SERPINB1 Antibody LS‑C482550
SERPINB1 antibody LS-C482550 is an unconjugated rabbit polyclonal antibody to SERPINB1 from human, mouse and rat. Validated for ICC, IF and WB.
Catalog
Size
Price
LS-C482550-50
50 µl (1 mg/ml)
$255
LS-C482550-100
100 µl (1 mg/ml)
$295
LS-C482550-200
200 µl (1 mg/ml)
$375
Description
SERPINB1 antibody LS-C482550 is an unconjugated rabbit polyclonal antibody to SERPINB1 from human, mouse and rat. Validated for ICC, IF and WB.
Target
Human SERPINB1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (1:20 - 1:100)
  • Immunofluorescence (1:20 - 1:100)
  • Western blot (1:500 - 1:2000)
Immunogen
SERPINB1 antibody was raised against recombinant full length Human SERPINB1.
Specificity
Recognizes endogenous levels of SerpinB1 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About SERPINB1
P30740 NM_030666 NP_109591.1

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Images

Immunofluorescence

Immunofluorescent analysis of SerpinB1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunofluorescent analysis of SerpinB1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot

Western blot analysis of SerpinB1 expression in MCF7 (A); A549 (B); mouse lung (C); mouse spleen (D); rat spleen (E) whole cell lysates.
Western blot analysis of SerpinB1 expression in MCF7 (A); A549 (B); mouse lung (C); mouse spleen (D); rat spleen (E) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of SerpinB1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunofluorescent analysis of SerpinB1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot

Western blot analysis of SerpinB1 expression in MCF7 (A); A549 (B); mouse lung (C); mouse spleen (D); rat spleen (E) whole cell lysates.
Western blot analysis of SerpinB1 expression in MCF7 (A); A549 (B); mouse lung (C); mouse spleen (D); rat spleen (E) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of SerpinB1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunofluorescent analysis of SerpinB1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot

Western blot analysis of SerpinB1 expression in MCF7 (A); A549 (B); mouse lung (C); mouse spleen (D); rat spleen (E) whole cell lysates.
Western blot analysis of SerpinB1 expression in MCF7 (A); A549 (B); mouse lung (C); mouse spleen (D); rat spleen (E) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of SerpinB1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunofluorescent analysis of SerpinB1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot

Western blot analysis of SerpinB1 expression in MCF7 (A); A549 (B); mouse lung (C); mouse spleen (D); rat spleen (E) whole cell lysates.
Western blot analysis of SerpinB1 expression in MCF7 (A); A549 (B); mouse lung (C); mouse spleen (D); rat spleen (E) whole cell lysates.

Requested From: United States
Date Requested: 11/16/2018
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