Human SELE / CD62E / E-selectin
SELE | CD62E | ELAM | ESEL | E-selectin | LECAM2 | CD62E antigen | ELAM-1 | ELAM1 | Selectin E
(tested or 100% immunogen sequence identity)
- IHC - Frozen
- Functional Assay
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Reacts with E-selectin CD62-E, previous designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). Reacts with human endothelial cells activated with TNF-alpha, IL-1 or endotoxin. Reacts with cells transfected with the E-selectin gene. Inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.
Useful for Immunohistochemical staining of E-selectin expressing endothelial cells. It permits staining of in vitro cultured cells as well as frozen sections. The antibody can also be used for staining of chimpanzee, rhesus monkey, cynomolgus monkey and baboon endothelial cells expressing E-selectin. Furthermore the antibody can be used for immunoassays and adhesion inhibition studies for which the antibody is most efficient and prevents adhesion of leucocytes via Fc receptors. Immunohistology: It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100U/ml) or LPS (1 ug/ml) in tissue culture medium at 37?C. As negative control it is advised to use a control murine IgG1 antibody. For use in adhesion studies dilutions have to be made according to the amounts of E-Selectin to be inhibited. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
PBS, 0.02% Sodium Azide, 0.1% BSA
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only. Intended for use by laboratory professionals.
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About SELE / CD62E / E-selectin