Human SCRIB / Scribble

Goat

Human (tested or 100% immunogen sequence identity)

Human (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated

ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Unmodified

Peptide with sequence C-PEGPGKEKELPGQ, from the internal region of the protein sequence according to NP_874365.3; NP_056171.3.

Internal Region

• Immunofluorescence (10 µg/ml)
• Western blot (1 - 3 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:1000)

Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Approx 200-250kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178kDa according to NP_874365.3), The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10µg/ml.

Tris saline 0.5 mg/ml, 0.02% sodium azide, pH 7.3, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q14160 NCBI: NM_015356 NP_056171.3