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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C59284-100 100 µl (85 mg/ml) $435 
SARS-CoV NSP8 Antibody - Immunoprecipitation followed by western blotting using Anti-nsp8 shows a predominant band at 21.8 kDa corresponding to full length SARS protein (panel A). Immunofluorescence Microscopy using anti-nsp8 6-h post infection of Vero-E6 cells (Panel B). For detection Cy3 conjugated Goat-anti-Rabbit IgG MX (611-104-122) was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.

Polyclonal Rabbit anti‑Coronavirus SARS‑CoV NSP8 Antibody (IF, WB) LS‑C59284

Polyclonal Rabbit anti‑Coronavirus SARS‑CoV NSP8 Antibody (IF, WB) LS‑C59284

Antibody:
SARS-CoV NSP8 Rabbit anti-Coronavirus SARS-CoV Polyclonal Antibody
Application:
IF, WB, IP, EM
Reactivity:
Coronavirus SARS-CoV
Format:
Unconjugated, Unmodified
Price
Catalog Number
$435
LS-C59284-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
SARS-CoV NSP8 Rabbit anti-Coronavirus SARS-CoV Polyclonal Antibody
Application:
IF, WB, IP, EM
Reactivity:
Coronavirus SARS-CoV
Format:
Unconjugated, Unmodified

Specifications

Description
SARS-CoV NSP8 antibody LS-C59284 is an unconjugated rabbit polyclonal antibody to coronavirus sars-cov NSP8. Validated for EM, IF, IP and WB.
Target
Coronavirus SARS-CoV SARS-CoV NSP8
Host
Rabbit
Reactivity
Coronavirus SARS-CoV (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
This whole rabbit serum was produced by repeated immunizations with a purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.
Specificity
This antibody is directed against SARS-Coronavirus nsp8 protein. The product is neat antiserum. Cross reactivity with homologues from other sources has not been determined.
Applications
  • Immunofluorescence (1:300)
  • Western blot (1:1000)
  • Immunoprecipitation (1:60)
  • Electron Microscopy
Usage
This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kD in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37?C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min. IMMUNO PRECIPITATION 1:60; IMMUNO ELECTRON MICROSCOPY 1:100;
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 9/24/2020