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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C59284-100 100 µl (85 mg/ml) $425 
SARS NSP8 Antibody - Immunoprecipitation followed by western blotting using Anti-nsp8 shows a predominant band at 21.8 kDa corresponding to full length SARS protein (panel A). Immunofluorescence Microscopy using anti-nsp8 6-h post infection of Vero-E6 cells (Panel B). For detection Cy3 conjugated Goat-anti-Rabbit IgG MX (611-104-122) was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.

SARS NSP8 Antibody LS‑C59284

SARS NSP8 Antibody LS‑C59284

Antibody:
Rabbit Polyclonal to SARS Coronavirus SARS NSP8
Application:
IF, WB, IP
Reactivity:
SARS Coronavirus
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C59284-100
100 µl
85 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to SARS Coronavirus SARS NSP8
Application:
IF, WB, IP
Reactivity:
SARS Coronavirus
Format:
Unconjugated, Unmodified

Specifications

Description
SARS NSP8 antibody LS-C59284 is an unconjugated rabbit polyclonal antibody to sars coronavirus SARS NSP8. Validated for IF, IP and WB.
Target
SARS Coronavirus SARS NSP8
Host
Rabbit
Reactivity
SARS Coronavirus (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • Immunofluorescence (1:300)
  • Western blot (1:1000)
  • Immunoprecipitation
Immunogen
Purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.
Specificity
SARS-Coronavirus nsp8 protein. Cross reactivity with homologs from other sources has not been determined
Usage
This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kD in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37?C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min. IMMUNO PRECIPITATION 1:60; IMMUNO ELECTRON MICROSCOPY 1:100;
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/22/2019