Coronavirus SARS-CoV SARS-CoV Matrix
(tested or 100% immunogen sequence identity)
- Immunofluorescence (1:1000)
- Western blot (1:1000)
- Immunoprecipitation (1:60)
- Electron Microscopy
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a BSA-coupled synthetic peptide corresponding to the C-terminus (amino acid residues 204-221) of the SARS Coronavirus Membrane protein.
This antibody is directed against SARS-Coronavirus membrane protein. The product is neat antiserum. Cross reactivity with homologues from other sources has not been determined.
This antibody has been tested for use in immunofluorescence microscopy, immunoprecipitation, immunoelectron microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 25 kD in size corresponding to SARS-CoV membrane protein by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37?C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min. IMMUNO PRECIPITATION 1:60; IMMUNO ELECTRON MICROSCOPY 1:200;
Antiserum, 0.01% Sodium Azide
Short term: -20°C; Long term: -20°C.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.