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Catalog Number Size Price
LS-C761879-100 100 µg (1 mg/ml) $574 
S100B / S100 Beta Antibody - IHC testing of FFPE human melanoma with S100 beta antibody (clone S100B/1012). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
S100B / S100 Beta Antibody - IHC testing of FFPE human schwanoma with S100 beta antibody (clone S100B/1012). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
S100B / S100 Beta Antibody - Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using S100 beta antibody (clone S100B/1012). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
S100B / S100 Beta Antibody - (Left) Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100 beta antibody (green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Right) Negative control.
S100B / S100 Beta Antibody - SDS-PAGE Analysis of Purified, BSA-Free S100 beta Antibody (clone S100B/1012). Confirmation of Integrity and Purity of the Antibody.
S100B / S100 Beta Antibody - IHC testing of FFPE human melanoma with S100 beta antibody (clone S100B/1012). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
S100B / S100 Beta Antibody - IHC testing of FFPE human schwanoma with S100 beta antibody (clone S100B/1012). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
S100B / S100 Beta Antibody - Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using S100 beta antibody (clone S100B/1012). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
S100B / S100 Beta Antibody - (Left) Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100 beta antibody (green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Right) Negative control.
S100B / S100 Beta Antibody - SDS-PAGE Analysis of Purified, BSA-Free S100 beta Antibody (clone S100B/1012). Confirmation of Integrity and Purity of the Antibody.
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Monoclonal Mouse anti‑Human S100B / S100 Beta Antibody (clone S100B/1012, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C761879

Monoclonal Mouse anti‑Human S100B / S100 Beta Antibody (clone S100B/1012, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C761879

Antibody:
S100B / S100 Beta Mouse anti-Human Monoclonal (Azide-free, BSA-free) (S100B/1012) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Price
Catalog Number
$574
LS-C761879-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
S100B / S100 Beta Mouse anti-Human Monoclonal (Azide-free, BSA-free) (S100B/1012) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:

Specifications

Description
S100 Beta antibody LS-C761879 is an unconjugated mouse monoclonal antibody to human S100 Beta (S100B). Validated for Flow, IF, IHC and WB.
Target
Human S100B / S100 Beta
Synonyms
S100B | NEF | Protein S100-B | S100 | S100 calcium binding protein B | S100 calcium-binding protein B | S100beta | S-100 protein beta chain | S-100 protein subunit beta | S100-B
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
S100B/1012
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Azide-free, BSA-free. Also available Unmodified or Ready-to-Use.
Immunogen
Full length recombinant human protein.
Specificity
This antibody has been extensively validated, including cross-reactivity testing against 19,000 full-length proteins by HuProt(TM) microarray analysis.
Applications
  • IHC - Paraffin (0.25 - 0.5 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Optimal dilution of the S100 beta antibody should be determined by the researcher. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Prediluted IHC only format: incubate for 30 min at RT
Presentation
1X PBS
Storage
Store at 2-8°C (with azide) or aliquot and store at -20°C (without azide).
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About S100B / S100 Beta
P04271 NM_006272 NP_006263.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/2/2024