MRP14 antibody LS-C96228 is an unconjugated mouse monoclonal antibody to human MRP14 (S100A9). Validated for ELISA and IHC.
(tested or 100% immunogen sequence identity)
S100A9 / MRP14 antibody was raised against cultured human monocytes
The antigen is MRP14, the epitope is suspected in the carboxyterminal portion of the peptide. Antigen distribution: Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from all other blood cells. In cultured monocytes, maximum MRP14 expression is found after 3 - 4 days. Myeloid leukaemia cells have been found to be positive as well. Tissue sections: MRP14 is found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP14 increasingly during the early stages of inflammation. A high MRP14 (and low MRP8) expression by macrophages was reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases like chronic rheumatoid arthritis, MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8/14 determined by antibody 27E10. Specificity: Human: MRP14, granulocytes, stimulated monocytes and macrophages. Other: does not react with bovine and swine spleen
Lyophilized from PBS pH 7.2, 5mg/ml BSA, and 0.05% Kathon CG